Comparison of the DiversiLab (TM) system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains

Rene Witt, V Kanhai, Willem Leeuwen

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Abstract

We have analyzed a representative selection of the HARMONY meticillin-resistant Staphylococcus aureus strain collection originating from 11 European countries (Cookson, B.D. et al., 2007, J. Clin. Microbiol. 45: 1830-1837) with the DiversiLab (TM) System, Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). Simpson's diversity indices were 0.905, 0.877 and 0.860 for PFGE, MLST and DiversiLab (TM), respectively. All methods displayed concordant classification of the MRSA strains, although with divergent resolution and reproducibility. (C) 2009 Elsevier B.V. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)130-133
Number of pages4
JournalJournal of Microbiological Methods
Volume77
Issue number1
DOIs
Publication statusPublished - 2009

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