Comparison of the DiversiLab (TM) system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains

Rene Witt; V Kanhai; Willem Leeuwen

doi:10.1016/j.mimet.2009.01.009

Comparison of the DiversiLab (TM) system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains

Rene Witt, V Kanhai, Willem Leeuwen

Medical Microbiology & Infectious Diseases

Research output: Contribution to journal › Article › Academic › peer-review

33 Citations (Scopus)

Abstract

We have analyzed a representative selection of the HARMONY meticillin-resistant Staphylococcus aureus strain collection originating from 11 European countries (Cookson, B.D. et al., 2007, J. Clin. Microbiol. 45: 1830-1837) with the DiversiLab (TM) System, Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). Simpson's diversity indices were 0.905, 0.877 and 0.860 for PFGE, MLST and DiversiLab (TM), respectively. All methods displayed concordant classification of the MRSA strains, although with divergent resolution and reproducibility. (C) 2009 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	130-133
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2009.01.009
Publication status	Published - 2009

Research programs

EMC MM-04-28-01

Access to Document

10.1016/j.mimet.2009.01.009

http://hdl.handle.net/1765/17677

Cite this

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title = "Comparison of the DiversiLab (TM) system, Pulsed-Field Gel Electrophoresis and Multi-Locus Sequence Typing for the characterization of epidemic reference MRSA strains",

abstract = "We have analyzed a representative selection of the HARMONY meticillin-resistant Staphylococcus aureus strain collection originating from 11 European countries (Cookson, B.D. et al., 2007, J. Clin. Microbiol. 45: 1830-1837) with the DiversiLab (TM) System, Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). Simpson's diversity indices were 0.905, 0.877 and 0.860 for PFGE, MLST and DiversiLab (TM), respectively. All methods displayed concordant classification of the MRSA strains, although with divergent resolution and reproducibility. (C) 2009 Elsevier B.V. All rights reserved.",

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AB - We have analyzed a representative selection of the HARMONY meticillin-resistant Staphylococcus aureus strain collection originating from 11 European countries (Cookson, B.D. et al., 2007, J. Clin. Microbiol. 45: 1830-1837) with the DiversiLab (TM) System, Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). Simpson's diversity indices were 0.905, 0.877 and 0.860 for PFGE, MLST and DiversiLab (TM), respectively. All methods displayed concordant classification of the MRSA strains, although with divergent resolution and reproducibility. (C) 2009 Elsevier B.V. All rights reserved.

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