Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

Tineke Herremans; Boris M. Hogema; Marrigje Nabuurs; Marcel Peeters; Marjolijn Wegdam-Blans; Peter Schneeberger; Carla Nijhuis; Daan W. Notermans; Joep Galama; Anton Horrevorts; Inge H.M. van Loo; Bart Vlaminckx; Hans L. Zaaijer; Marion P. Koopmans; Hanneke Berkhout; Cristina Socolovschi; Didier Raoult; John Stenos; William Nicholson; Henk Bijlmer

doi:10.1016/j.diagmicrobio.2012.09.001

Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

Tineke Herremans^*
, Boris M. Hogema
, Marrigje Nabuurs
, Marcel Peeters
, Marjolijn Wegdam-Blans
, Peter Schneeberger
, Carla Nijhuis
, Daan W. Notermans
, Joep Galama
, Anton Horrevorts
, Inge H.M. van Loo
, Bart Vlaminckx
, Hans L. Zaaijer
, Marion P. Koopmans
, Hanneke Berkhout
, Cristina Socolovschi
, Didier Raoult
, John Stenos
, William Nicholson
, Henk Bijlmer

^*Corresponding author for this work

National Institute of Public Health and the Environment
Radboud University Medical Center
Canisius Wilhelmina Hospital
ETZ Elisabeth
Laboratory for Pathology and Medical Microbiology (PAMM)
Jeroen Bosch Ziekenhuis
Maastricht University
St. Antonius Ziekenhuis
Sanquin Blood Supply Foundation
VieCuri Medisch Centrum
Unité des Rickettsies
Australian Rickettsial Reference Laboratory
Centers for Disease Control and Prevention

Research output: Contribution to journal › Article › Academic › peer-review

31 Citations (Scopus)

Abstract

The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories using the same methods were within close range, and all 3 methods correctly identified acute Q fever patients. The IFA, ELISA, and CFA are all suitable serodiagnostic assays to diagnose acute Q fever, but the IFA remains an important tool in the follow-up of patients and in identifying patients at risk for developing chronic Q fever.

Original language	English
Pages (from-to)	16-21
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.09.001
Publication status	Published - Jan 2013
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.1016/j.diagmicrobio.2012.09.001

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Herremans, T., Hogema, B. M., Nabuurs, M., Peeters, M., Wegdam-Blans, M., Schneeberger, P., Nijhuis, C., Notermans, D. W., Galama, J., Horrevorts, A., van Loo, I. H. M., Vlaminckx, B., Zaaijer, H. L., Koopmans, M. P., Berkhout, H., Socolovschi, C., Raoult, D., Stenos, J., Nicholson, W., & Bijlmer, H. (2013). Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment. Diagnostic Microbiology and Infectious Disease, 75(1), 16-21. https://doi.org/10.1016/j.diagmicrobio.2012.09.001

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title = "Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment",

abstract = "The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories using the same methods were within close range, and all 3 methods correctly identified acute Q fever patients. The IFA, ELISA, and CFA are all suitable serodiagnostic assays to diagnose acute Q fever, but the IFA remains an important tool in the follow-up of patients and in identifying patients at risk for developing chronic Q fever.",

author = "Tineke Herremans and Hogema, \{Boris M.\} and Marrigje Nabuurs and Marcel Peeters and Marjolijn Wegdam-Blans and Peter Schneeberger and Carla Nijhuis and Notermans, \{Daan W.\} and Joep Galama and Anton Horrevorts and \{van Loo\}, \{Inge H.M.\} and Bart Vlaminckx and Zaaijer, \{Hans L.\} and Koopmans, \{Marion P.\} and Hanneke Berkhout and Cristina Socolovschi and Didier Raoult and John Stenos and William Nicholson and Henk Bijlmer",

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doi = "10.1016/j.diagmicrobio.2012.09.001",

language = "English",

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Herremans, T, Hogema, BM, Nabuurs, M, Peeters, M, Wegdam-Blans, M, Schneeberger, P, Nijhuis, C, Notermans, DW, Galama, J, Horrevorts, A, van Loo, IHM, Vlaminckx, B, Zaaijer, HL, Koopmans, MP, Berkhout, H, Socolovschi, C, Raoult, D, Stenos, J, Nicholson, W & Bijlmer, H 2013, 'Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment', Diagnostic Microbiology and Infectious Disease, vol. 75, no. 1, pp. 16-21. https://doi.org/10.1016/j.diagmicrobio.2012.09.001

TY - JOUR

T1 - Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

AU - Herremans, Tineke

AU - Hogema, Boris M.

AU - Nabuurs, Marrigje

AU - Peeters, Marcel

AU - Wegdam-Blans, Marjolijn

AU - Schneeberger, Peter

AU - Nijhuis, Carla

AU - Notermans, Daan W.

AU - Galama, Joep

AU - Horrevorts, Anton

AU - van Loo, Inge H.M.

AU - Vlaminckx, Bart

AU - Zaaijer, Hans L.

AU - Koopmans, Marion P.

AU - Berkhout, Hanneke

AU - Socolovschi, Cristina

AU - Raoult, Didier

AU - Stenos, John

AU - Nicholson, William

AU - Bijlmer, Henk

PY - 2013/1

Y1 - 2013/1

N2 - The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories using the same methods were within close range, and all 3 methods correctly identified acute Q fever patients. The IFA, ELISA, and CFA are all suitable serodiagnostic assays to diagnose acute Q fever, but the IFA remains an important tool in the follow-up of patients and in identifying patients at risk for developing chronic Q fever.

AB - The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories using the same methods were within close range, and all 3 methods correctly identified acute Q fever patients. The IFA, ELISA, and CFA are all suitable serodiagnostic assays to diagnose acute Q fever, but the IFA remains an important tool in the follow-up of patients and in identifying patients at risk for developing chronic Q fever.

UR - https://www.scopus.com/pages/publications/84870733369

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DO - 10.1016/j.diagmicrobio.2012.09.001

M3 - Article

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AN - SCOPUS:84870733369

SN - 0732-8893

VL - 75

SP - 16

EP - 21

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

Abstract

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