Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing

S Gombar; HJ Jung; F Dong; B Calder; G Atzmon; N Barzilai; XL Tian; Joris Pothof; Jan Hoeijmakers; J Campisi; J Vijg; Y Suh

doi:10.1186/1471-2164-13-353

Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing

S Gombar
, HJ Jung
, F Dong
, B Calder
, G Atzmon
, N Barzilai
, XL Tian
, Joris Pothof
, Jan Hoeijmakers
, J Campisi
, J Vijg
, Y Suh

Molecular Genetics

Research output: Contribution to journal › Article › Academic › peer-review

73 Citations (Scopus)

49 Downloads (Pure)

Abstract

Background: MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and play a critical role in development, homeostasis, and disease. Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity. Results: We employed massively parallel sequencing technology to identify miRNAs expressed in B-cells from Ashkenazi Jewish centenarians, i.e., those living to a hundred and a human model of exceptional longevity, and younger controls without a family history of longevity. With data from 26.7 million reads comprising 9.4 x 10(8) bp from 3 centenarian and 3 control individuals, we discovered a total of 276 known miRNAs and 8 unknown miRNAs ranging several orders of magnitude in expression levels, Conclusion: Our comprehensive miRNA data provide a resource for further studies to identify genetic pathways associated with aging and longevity in humans.

Original language	Undefined/Unknown
Journal	BMC Genomics
Volume	13
Issue number	353
DOIs	https://doi.org/10.1186/1471-2164-13-353
Publication status	Published - 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research programs

EMC MGC-01-12-03

Access to Document

10.1186/1471-2164-13-353

REPUB_74659_OA.pdfFinal published version, 1.07 MB

http://hdl.handle.net/1765/74659

Cite this

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title = "Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing",

abstract = "Background: MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and play a critical role in development, homeostasis, and disease. Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity. Results: We employed massively parallel sequencing technology to identify miRNAs expressed in B-cells from Ashkenazi Jewish centenarians, i.e., those living to a hundred and a human model of exceptional longevity, and younger controls without a family history of longevity. With data from 26.7 million reads comprising 9.4 x 10(8) bp from 3 centenarian and 3 control individuals, we discovered a total of 276 known miRNAs and 8 unknown miRNAs ranging several orders of magnitude in expression levels, Conclusion: Our comprehensive miRNA data provide a resource for further studies to identify genetic pathways associated with aging and longevity in humans.",

author = "S Gombar and HJ Jung and F Dong and B Calder and G Atzmon and N Barzilai and XL Tian and Joris Pothof and Jan Hoeijmakers and J Campisi and J Vijg and Y Suh",

year = "2012",

doi = "10.1186/1471-2164-13-353",

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volume = "13",

journal = "BMC Genomics",

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T1 - Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing

AU - Gombar, S

AU - Jung, HJ

AU - Dong, F

AU - Calder, B

AU - Atzmon, G

AU - Barzilai, N

AU - Tian, XL

AU - Pothof, Joris

AU - Hoeijmakers, Jan

AU - Campisi, J

AU - Vijg, J

AU - Suh, Y

PY - 2012

Y1 - 2012

N2 - Background: MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and play a critical role in development, homeostasis, and disease. Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity. Results: We employed massively parallel sequencing technology to identify miRNAs expressed in B-cells from Ashkenazi Jewish centenarians, i.e., those living to a hundred and a human model of exceptional longevity, and younger controls without a family history of longevity. With data from 26.7 million reads comprising 9.4 x 10(8) bp from 3 centenarian and 3 control individuals, we discovered a total of 276 known miRNAs and 8 unknown miRNAs ranging several orders of magnitude in expression levels, Conclusion: Our comprehensive miRNA data provide a resource for further studies to identify genetic pathways associated with aging and longevity in humans.

AB - Background: MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and play a critical role in development, homeostasis, and disease. Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity. Results: We employed massively parallel sequencing technology to identify miRNAs expressed in B-cells from Ashkenazi Jewish centenarians, i.e., those living to a hundred and a human model of exceptional longevity, and younger controls without a family history of longevity. With data from 26.7 million reads comprising 9.4 x 10(8) bp from 3 centenarian and 3 control individuals, we discovered a total of 276 known miRNAs and 8 unknown miRNAs ranging several orders of magnitude in expression levels, Conclusion: Our comprehensive miRNA data provide a resource for further studies to identify genetic pathways associated with aging and longevity in humans.

U2 - 10.1186/1471-2164-13-353

DO - 10.1186/1471-2164-13-353

M3 - Article

C2 - 22846614

SN - 1471-2164

VL - 13

JO - BMC Genomics

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IS - 353

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