Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome

XG Yang; Y Levin; H Rahmoune; D Ma; S Schoffmann; Y Umrania; PC Guest; Sabine Bahn

doi:10.1002/pmic.201000525

Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome

XG Yang, Y Levin, H Rahmoune, D Ma, S Schoffmann, Y Umrania, PC Guest, Sabine Bahn

Neurosciences

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator (R) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.

Original language	Undefined/Unknown
Pages (from-to)	501-505
Number of pages	5
Journal	Proteomics
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/pmic.201000525
Publication status	Published - 2011

Access to Document

10.1002/pmic.201000525

http://hdl.handle.net/1765/31699

Cite this

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AU - Levin, Y

AU - Rahmoune, H

AU - Ma, D

AU - Schoffmann, S

AU - Umrania, Y

AU - Guest, PC

AU - Bahn, Sabine

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AB - In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator (R) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.

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