Construction and functional analysis of hybrid interleukin-6 variants Characterization of the role of the C-terminus for species specificity

Frank W.G. Leebeek; Dana M. Fowlkes

doi:10.1016/0014-5793(92)81013-C

Construction and functional analysis of hybrid interleukin-6 variants Characterization of the role of the C-terminus for species specificity

Frank W.G. Leebeek^*, Dana M. Fowlkes

^*Corresponding author for this work

Internal Medicine

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6, IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays in important role in the species specificity of IL-6.

Original language	English
Pages (from-to)	262-264
Number of pages	3
Journal	FEBS Letters
Volume	306
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(92)81013-C
Publication status	Published - 20 Jul 1992

Bibliographical note

Access to Document

10.1016/0014-5793(92)81013-C

Cite this

@article{d2a2170d26434d0198ed6b3e8a49a964,

title = "Construction and functional analysis of hybrid interleukin-6 variants Characterization of the role of the C-terminus for species specificity",

abstract = "We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6, IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays in important role in the species specificity of IL-6.",

author = "Leebeek, {Frank W.G.} and Fowlkes, {Dana M.}",

note = "{\textcopyright} 1992 Federation of European Biochemical Societies",

year = "1992",

month = jul,

day = "20",

doi = "10.1016/0014-5793(92)81013-C",

language = "English",

volume = "306",

pages = "262--264",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "2-3",

}

TY - JOUR

T1 - Construction and functional analysis of hybrid interleukin-6 variants Characterization of the role of the C-terminus for species specificity

AU - Leebeek, Frank W.G.

AU - Fowlkes, Dana M.

PY - 1992/7/20

Y1 - 1992/7/20

N2 - We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6, IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays in important role in the species specificity of IL-6.

AB - We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6, IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays in important role in the species specificity of IL-6.

UR - http://www.scopus.com/inward/record.url?scp=0026776544&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(92)81013-C

DO - 10.1016/0014-5793(92)81013-C

M3 - Article

C2 - 1633883

AN - SCOPUS:0026776544

SN - 0014-5793

VL - 306

SP - 262

EP - 264

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Construction and functional analysis of hybrid interleukin-6 variants Characterization of the role of the C-terminus for species specificity

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this