Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning

Alessandro Dema, Shima Rahgozar, Laurent Siquier, Jeffrey van Haren, Torsten Wittmann*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Micropatterning of extracellular matrix proteins enables defining cell position and shape in experiments investigating intracellular dynamics and organization. While such standardization is advantageous in automated and quantitative analysis of many cells, the original methods generating such patterns are cumbersome and inflexible. However, recent development of contact-less methods that allow photochemical generation of protein patterns robustly and rapidly is boosting the broader availability of micropatterning approaches. Here, we describe an optimized protocol to achieve large micropatterned areas with high fidelity using a commercially available microscope-mounted UV projection system.

Original languageEnglish
Pages (from-to)467-481
Number of pages15
JournalMethods in Molecular Biology
Volume2430
Early online date28 Apr 2022
DOIs
Publication statusPublished - 2022
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by NIH grant R01 NS107480 to T.W. and funding by the UCSF Strategic Initiative for the ‘Micro-Maker Lab’.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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