Coupling Two Ultra-high-Speed Cameras to Elucidate Ultrasound Contrast-Mediated Imaging and Therapy

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Abstract

Ultrasound contrast-mediated medical imaging and therapy both rely on the dynamics of micron- and nanometer-sized ultrasound cavitation nuclei, such as phospholipid-coated microbubbles and phase-change droplets. Ultrasound cavitation nuclei respond non-linearly to ultrasound on a nanosecond time scale that necessitates the use of ultra-high-speed imaging to fully visualize these dynamics in detail. In this study, we developed an ultra-high-speed optical imaging system that can record up to 20 million frames per second (Mfps) by coupling two small-sized, commercially available, 10-Mfps cameras. The timing and reliability of the interleaved cameras needed to achieve 20 Mfps was validated using two synchronized light-emitting diode strobe lights. Once verified, ultrasound-activated microbubble responses were recorded and analyzed. A unique characteristic of this coupled system is its ability to be reconfigured to provide orthogonal observations at 10 Mfps. Acoustic droplet vaporization was imaged from two orthogonal views, by which the 3-D dynamics of the phase transition could be visualized. This optical imaging system provides the temporal resolution and experimental flexibility needed to further elucidate the dynamics of ultrasound cavitation nuclei to potentiate the clinical translation of ultrasound-mediated imaging and therapy developments.

Original languageEnglish
Pages (from-to)388-397
Number of pages10
JournalUltrasound in Medicine and Biology
Volume49
Issue number1
Early online date12 Oct 2022
DOIs
Publication statusPublished - 1 Jan 2023

Bibliographical note

Funding Information:
This work was funded by the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Program (Grant Agreement 805308). The authors thank Bram Meijlink (Erasmus MC) for microbubble production, Michiel Manten (Erasmus MC) for designing and manufacturing the orthogonal apparatus and Hendrik J. Vos (Erasmus MC) for the fruitful discussions on the orthogonal experiments. The authors declare no known conflicts of interest.

Funding Information:
This work was funded by the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Program (Grant Agreement 805308). The authors thank Bram Meijlink (Erasmus MC) for microbubble production, Michiel Manten (Erasmus MC) for designing and manufacturing the orthogonal apparatus and Hendrik J. Vos (Erasmus MC) for the fruitful discussions on the orthogonal experiments.

Publisher Copyright:
© 2022 The Authors

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