Cutaneous respirometry by dynamic measurement of mitochondrial oxygen tension for monitoring mitochondrial function in vivo

Floor Harms, Jacqueline Voorbeijtel, Sander Bodmer, Harold Raat, Bert Mik

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Abstract

Progress in diagnosis and treatment of mitochondrial dysfunction in chronic and acute disease could greatly benefit from techniques for monitoring of mitochondrial function in vivo. In this study we demonstrate the feasibility of in vivo respirometry in skin. Mitochondrial oxygen measurements by means of oxygen-dependent delayed fluorescence of protoporphyrin IX are shown to provide a robust basis for measurement of local oxygen disappearance rate (ODR). The fundamental principles behind the technology are described, together with an analysis method for retrievel of respirometry data. The feasibility and reproducibility of this clinically useful approach are demonstrated in a series of rats. (C) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)507-514
Number of pages8
JournalMitochondrion
Volume13
Issue number5
DOIs
Publication statusPublished - 2013

Research programs

  • EMC COEUR-09

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