Cytocam-IDF (incident dark field illumination) imaging for bedside monitoring of the microcirculation

Guclu Aykut, Gerke Veenstra*, Claudia Scorcella, Can Ince, Christiaan Boerma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

171 Citations (Scopus)
21 Downloads (Pure)


Background: Orthogonal polarized spectral (OPS) and sidestream dark field (SDF)
imaging video microscope devices were introduced for observation of the
microcirculation but, due to technical limitations, have remained as research tools.
Recently, a novel handheld microscope based on incident dark field illumination
(IDF) has been introduced for clinical use. The Cytocam-IDF imaging device consists
of a pen-like probe incorporating IDF illumination with a set of high-resolution lenses
projecting images on to a computer controlled image sensor synchronized with very
short pulsed illumination light. This study was performed to validate Cytocam-IDF
imaging by comparison to SDF imaging in volunteers.
Methods: This study is a prospective, observational study. The subjects consist of 25
Results: Sublingual microcirculation was evaluated using both techniques. The main
result was that Cytocam-IDF imaging provided better quality images and was able to
detect 30% more capillaries than SDF imaging (total vessels density Cytocam-IDF:
21.60 ± 4.30 mm/mm2 vs SDF: 16.35 ± 2.78 mm/mm2
, p < 0.0001). Comparison of
the images showed increased contrast, sharpness, and image quality of both venules
and capillaries.
Conclusions: Cytocam-IDF imaging detected more capillaries and provided better
image quality than SDF imaging. It is concluded that Cytocam-IDF imaging may
provide a new improved imaging modality for clinical assessment of microcirculatory
Keywords: Microcirculation; SDF imaging; Cytocam-IDF imaging; İntravital microscopy;
Original languageEnglish
Number of pages10
JournalIntensive Care Medicine Experimental
Publication statusPublished - 31 Jan 2015


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