TY - JOUR
T1 - Description of mitochondrial oxygen tension and its variability in healthy volunteers
AU - Baysan, Meryem
AU - Broere, Mark
AU - Wille, Maarten E.
AU - Bergsma, Jule E.
AU - Mik, Egbert G.
AU - Juffermans, Nicole P.
AU - Tsonaka, Roula
AU - van der Bom, Johanna G.
AU - Arbous, Sesmu M.
N1 - Publisher Copyright: Copyright: © 2024 Baysan et al.
PY - 2024/6/3
Y1 - 2024/6/3
N2 - Objectives Describing mitochondrial oxygenation (mitoPO2) and its within- and between-subject variability over time after 5-aminolevulinic acid (ALA) plaster application in healthy volunteers. Design Prospective cohort study. Setting Measurements were performed in Leiden University Medical Center, the Netherlands. Participants Healthy volunteers enrolled from July to September 2020. Interventions Two ALA plasters were placed parasternal left and right, with a 3-hour time interval, to examine the influence of the calendar time on the value of mitoPO2. We measured mitoPO2 at 4, 5, 7, 10, 28, and 31 hours after ALA plaster 1 application, and at 4, 5, 7, 25, and 28 hours after ALA plaster 2 application. Primary and secondary outcome measures At each time point, five mitoPO2 measurements were performed. Within-subject variability was defined as the standard deviation (SD) of the mean of five measurements per timepoint of a study participant. The between-subject variability was the SD of the mean mitoPO2 value of the study population per timepoint. Results In 16 completed inclusions, median mitoPO2 values and within-subject variability were relatively similar over time at all time points for both plasters. An increase in overall between-subject variability was seen after 25 hours ALA plaster time (19.6 mm Hg vs 23.9 mm Hg after respectively 10 and 25 hours ALA plaster time). Conclusions The mitoPO2 values and within-subject variability remained relatively stable over time in healthy volunteers. An increase in between-subject variability was seen after 25 hours ALA plaster time warranting replacement of the ALA plaster one day after its application. Trial registration ClinicalTrials.gov with trial number NCT04626661.
AB - Objectives Describing mitochondrial oxygenation (mitoPO2) and its within- and between-subject variability over time after 5-aminolevulinic acid (ALA) plaster application in healthy volunteers. Design Prospective cohort study. Setting Measurements were performed in Leiden University Medical Center, the Netherlands. Participants Healthy volunteers enrolled from July to September 2020. Interventions Two ALA plasters were placed parasternal left and right, with a 3-hour time interval, to examine the influence of the calendar time on the value of mitoPO2. We measured mitoPO2 at 4, 5, 7, 10, 28, and 31 hours after ALA plaster 1 application, and at 4, 5, 7, 25, and 28 hours after ALA plaster 2 application. Primary and secondary outcome measures At each time point, five mitoPO2 measurements were performed. Within-subject variability was defined as the standard deviation (SD) of the mean of five measurements per timepoint of a study participant. The between-subject variability was the SD of the mean mitoPO2 value of the study population per timepoint. Results In 16 completed inclusions, median mitoPO2 values and within-subject variability were relatively similar over time at all time points for both plasters. An increase in overall between-subject variability was seen after 25 hours ALA plaster time (19.6 mm Hg vs 23.9 mm Hg after respectively 10 and 25 hours ALA plaster time). Conclusions The mitoPO2 values and within-subject variability remained relatively stable over time in healthy volunteers. An increase in between-subject variability was seen after 25 hours ALA plaster time warranting replacement of the ALA plaster one day after its application. Trial registration ClinicalTrials.gov with trial number NCT04626661.
UR - http://www.scopus.com/inward/record.url?scp=85195015423&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0300602
DO - 10.1371/journal.pone.0300602
M3 - Article
C2 - 38829894
AN - SCOPUS:85195015423
SN - 1932-6203
VL - 19
JO - PLoS ONE
JF - PLoS ONE
IS - 6 June
M1 - e0300602
ER -