Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating

Ioannis Androulakis; Riccardo Ferrero; Rogier van Oossanen; Alessandra Manzin; Antonia G Denkova; Kristina Djanashvili; Robin Nadar; Gerard C van Rhoon

doi:10.3390/s23094514

Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating

Ioannis Androulakis, Riccardo Ferrero, Rogier van Oossanen, Alessandra Manzin, Antonia G Denkova, Kristina Djanashvili, Robin Nadar, Gerard C van Rhoon^*

^*Corresponding author for this work

Radiotherapy

Research output: Contribution to journal › Article › Academic › peer-review

32 Downloads (Pure)

Abstract

While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose-effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale.

Original language	English
Article number	4514
Journal	Sensors (Basel, Switzerland)
Volume	23
Issue number	9
DOIs	https://doi.org/10.3390/s23094514
Publication status	Published - 5 May 2023

Bibliographical note

Funding Information:
This research was developed in the framework of Project 18HLT06 RaCHy, which received funding from the European Metrology Programme for Innovation and Research (EMPIR), co-financed by the participating states, and from the European Union’s Horizon 2020 Programme. K.D., A.G.D., G.C.v.R. and R.v.O. thank HTSM-NWO-TTW-project (grant nr. 16238) for financial support. I.A., R.v.O., R.N. and G.C.v.R. thank Elekta (grant nr. 106932) for financial support.

Publisher Copyright:
© 2023 by the authors.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/s23094514Licence: CC BY

Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced HeatingFinal published version, 1.59 MBLicence: CC BY

Cite this

@article{2a2ab06b671e41fc93897c93d11e60e2,

title = "Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating",

abstract = "While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose-effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale.",

author = "Ioannis Androulakis and Riccardo Ferrero and {van Oossanen}, Rogier and Alessandra Manzin and Denkova, {Antonia G} and Kristina Djanashvili and Robin Nadar and {van Rhoon}, {Gerard C}",

note = "Funding Information: This research was developed in the framework of Project 18HLT06 RaCHy, which received funding from the European Metrology Programme for Innovation and Research (EMPIR), co-financed by the participating states, and from the European Union{\textquoteright}s Horizon 2020 Programme. K.D., A.G.D., G.C.v.R. and R.v.O. thank HTSM-NWO-TTW-project (grant nr. 16238) for financial support. I.A., R.v.O., R.N. and G.C.v.R. thank Elekta (grant nr. 106932) for financial support. Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = may,

day = "5",

doi = "10.3390/s23094514",

language = "English",

volume = "23",

journal = "Sensors (Basel, Switzerland)",

issn = "1424-8220",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "9",

}

TY - JOUR

T1 - Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating

AU - Androulakis, Ioannis

AU - Ferrero, Riccardo

AU - van Oossanen, Rogier

AU - Manzin, Alessandra

AU - Denkova, Antonia G

AU - Djanashvili, Kristina

AU - Nadar, Robin

AU - van Rhoon, Gerard C

N1 - Funding Information: This research was developed in the framework of Project 18HLT06 RaCHy, which received funding from the European Metrology Programme for Innovation and Research (EMPIR), co-financed by the participating states, and from the European Union’s Horizon 2020 Programme. K.D., A.G.D., G.C.v.R. and R.v.O. thank HTSM-NWO-TTW-project (grant nr. 16238) for financial support. I.A., R.v.O., R.N. and G.C.v.R. thank Elekta (grant nr. 106932) for financial support. Publisher Copyright: © 2023 by the authors.

PY - 2023/5/5

Y1 - 2023/5/5

N2 - While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose-effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale.

AB - While hyperthermia has been shown to induce a variety of cytotoxic and sensitizing effects on cancer tissues, the thermal dose-effect relationship is still not well quantified, and it is still unclear how it can be optimally combined with other treatment modalities. Additionally, it is speculated that different methods of applying hyperthermia, such as water bath heating or electromagnetic energy, may have an effect on the resulting biological mechanisms involved in cell death or in sensitizing tumor cells to other oncological treatments. In order to further quantify and characterize hyperthermia treatments on a cellular level, in vitro experiments shifted towards the use of 3D cell spheroids. These are in fact considered a more representative model of the cell environment when compared to 2D cell cultures. In order to perform radiofrequency (RF)-induced heating in vitro, we have recently developed a dedicated electromagnetic field applicator. In this study, using this applicator, we designed and validated an experimental setup which can heat 3D cell spheroids in a conical polypropylene vial, thus providing a reliable instrument for investigating hyperthermia effects at the cellular scale.

UR - http://www.scopus.com/inward/record.url?scp=85159270622&partnerID=8YFLogxK

U2 - 10.3390/s23094514

DO - 10.3390/s23094514

M3 - Article

C2 - 37177718

SN - 1424-8220

VL - 23

JO - Sensors (Basel, Switzerland)

JF - Sensors (Basel, Switzerland)

IS - 9

M1 - 4514

ER -

Design and Validation of Experimental Setup for Cell Spheroid Radiofrequency-Induced Heating

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this