Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

VM Corman, I Eckerle, T Bleicker, A Zaki, O Landt, M Eschbach-Bludau, Sander van Boheemen, R Gopal, M Ballhause, Theo Bestebroer, D Muth, MA Muller, JF Drexler, M Zambon, Ab Osterhaus, RM Fouchier, C Drosten

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Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
Original languageUndefined/Unknown
Pages (from-to)3-8
Number of pages6
JournalEurosurveillance
Volume17
Issue number39
Publication statusPublished - 2012

Research programs

  • EMC MM-04-27-01

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