Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
Original language | Undefined/Unknown |
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Pages (from-to) | 3-8 |
Number of pages | 6 |
Journal | Eurosurveillance |
Volume | 17 |
Issue number | 39 |
Publication status | Published - 2012 |
Research programs
- EMC MM-04-27-01