Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

VM Corman; I Eckerle; T Bleicker; A Zaki; O Landt; M Eschbach-Bludau; Sander van Boheemen; R Gopal; M Ballhause; Theo Bestebroer; D Muth; MA Muller; JF Drexler; M Zambon; Ab Osterhaus; RM Fouchier; C Drosten

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

VM Corman, I Eckerle, T Bleicker, A Zaki, O Landt, M Eschbach-Bludau, Sander van Boheemen, R Gopal, M Ballhause, Theo Bestebroer, D Muth, MA Muller, JF Drexler, M Zambon, Ab Osterhaus, RM Fouchier, C Drosten

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Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Original language	Undefined/Unknown
Pages (from-to)	3-8
Number of pages	6
Journal	Eurosurveillance
Volume	17
Issue number	39
Publication status	Published - 2012

Research programs

EMC MM-04-27-01

Cite this

Corman, VM., Eckerle, I., Bleicker, T., Zaki, A., Landt, O., Eschbach-Bludau, M., van Boheemen, S., Gopal, R., Ballhause, M., Bestebroer, T., Muth, D., Muller, MA., Drexler, JF., Zambon, M., Osterhaus, A., Fouchier, RM., & Drosten, C. (2012). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Eurosurveillance, 17(39), 3-8.

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abstract = "We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.",

author = "VM Corman and I Eckerle and T Bleicker and A Zaki and O Landt and M Eschbach-Bludau and {van Boheemen}, Sander and R Gopal and M Ballhause and Theo Bestebroer and D Muth and MA Muller and JF Drexler and M Zambon and Ab Osterhaus and RM Fouchier and C Drosten",

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T1 - Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

AU - Corman, VM

AU - Eckerle, I

AU - Bleicker, T

AU - Zaki, A

AU - Landt, O

AU - Eschbach-Bludau, M

AU - van Boheemen, Sander

AU - Gopal, R

AU - Ballhause, M

AU - Bestebroer, Theo

AU - Muth, D

AU - Muller, MA

AU - Drexler, JF

AU - Zambon, M

AU - Osterhaus, Ab

AU - Fouchier, RM

AU - Drosten, C

PY - 2012

Y1 - 2012

N2 - We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

AB - We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

M3 - Article

SN - 1560-7917

VL - 17

SP - 3

EP - 8

JO - Eurosurveillance

JF - Eurosurveillance

IS - 39

ER -