Detection of SARS-CoV-2 in Air and on Surfaces in Rooms of Infected Nursing Home Residents

K. J. Linde*, I. M. Wouters, COCON Consortium, J. A. J. W. Kluytmans, M. F. Q. Kluytmans-van den Bergh, S. D. Pas, C. H. GeurtsvanKessel, M. P. G. Koopmans, M. Meier, P. Meijer, C. R. Raben, J. Spithoven, M. H. G. Tersteeg-Zijderveld, D. J. J. Heederik, W. Dohmen

*Corresponding author for this work

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There is an ongoing debate on airborne transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as a risk factor for infection. In this study, the level of SARS-CoV-2 in air and on surfaces of SARS-CoV-2 infected nursing home residents was assessed to gain insight in potential transmission routes. During outbreaks, air samples were collected using three different active and one passive air sampling technique in rooms of infected patients. Oropharyngeal swabs (OPS) of the residents and dry surface swabs were collected. Additionally, longitudinal passive air samples were collected during a period of 4 months in common areas of the wards. Presence of SARS-CoV-2 RNA was determined using RT-qPCR, targeting the RdRp- and E-genes. OPS, samples of two active air samplers and surface swabs with Ct-value 4 mu m 60% (6/10); 1-4 mu m 50% (5/10);

Original languageEnglish
Number of pages12
JournalAnnals of Work Exposures and Health
Publication statusE-pub ahead of print - 7 Sep 2022

Bibliographical note

Funding for this project was provided by ZonMw and part of Control of COVID-19 iN Hospitals (COCON) consortium
(project number 10150062010004).


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