Determinants of DNA yield and purity collected with buccal cell samples

DBMA Van Wieren-De Wijer, AH Zee, A den Boer, SV Belitser, André Kroon, PW de Leeuw, P Schiffers, RGJH Janssen, Cornelia Duijn, Bruno Stricker, OH Klungel

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)
5 Downloads (Pure)

Abstract

Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 mu g (median 54.3 mu g; mean 82.2 mu g +/- A SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 mu g) than those from women (median 44.2 mu g). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.
Original languageUndefined/Unknown
Pages (from-to)677-682
Number of pages6
JournalEuropean Journal of Epidemiology
Volume24
Issue number11
DOIs
Publication statusPublished - 2009

Cite this