Determination of propylene glycol in low volume plasma and urine samples of neonates by LC with photodiode array detection

Aida Kulo; Karel Allegaert; Jan De Hoon; Rene Verbesselt

doi:10.1007/s10337-011-1923-8

Determination of propylene glycol in low volume plasma and urine samples of neonates by LC with photodiode array detection

Aida Kulo^*
, Karel Allegaert
, Jan De Hoon
, Rene Verbesselt

^*Corresponding author for this work

University Hospitals Leuven
University of Sarajevo

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25-50 mg L^-1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15-46 mg L^-1) and urine samples (20-175 mg L^-1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates.

Original language	English
Pages (from-to)	463-470
Number of pages	8
Journal	Chromatographia
Volume	73
Issue number	5-6
DOIs	https://doi.org/10.1007/s10337-011-1923-8
Publication status	Published - Mar 2011
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments The clinical research of Karel Allegaert is supported by the Fund for Scientific Research, Flanders (Belgium) (F.W.O. Vlaanderen) by a Fundamental Clinical Investigatorship (1800209 N), and of Aida Kulo by a JoinEU-SEE scholarship (2009–2010). We would like to thank Sonia Demarsin for her technical assistance.

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title = "Determination of propylene glycol in low volume plasma and urine samples of neonates by LC with photodiode array detection",

abstract = "In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25-50 mg L-1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15-46 mg L-1) and urine samples (20-175 mg L-1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates.",

author = "Aida Kulo and Karel Allegaert and \{De Hoon\}, Jan and Rene Verbesselt",

note = "Funding Information: Acknowledgments The clinical research of Karel Allegaert is supported by the Fund for Scientific Research, Flanders (Belgium) (F.W.O. Vlaanderen) by a Fundamental Clinical Investigatorship (1800209 N), and of Aida Kulo by a JoinEU-SEE scholarship (2009{\textendash}2010). We would like to thank Sonia Demarsin for her technical assistance.",

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AU - Allegaert, Karel

AU - De Hoon, Jan

AU - Verbesselt, Rene

N1 - Funding Information: Acknowledgments The clinical research of Karel Allegaert is supported by the Fund for Scientific Research, Flanders (Belgium) (F.W.O. Vlaanderen) by a Fundamental Clinical Investigatorship (1800209 N), and of Aida Kulo by a JoinEU-SEE scholarship (2009–2010). We would like to thank Sonia Demarsin for her technical assistance.

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AB - In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25-50 mg L-1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15-46 mg L-1) and urine samples (20-175 mg L-1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates.

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JO - Chromatographia

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IS - 5-6

ER -

Determination of propylene glycol in low volume plasma and urine samples of neonates by LC with photodiode array detection

Abstract

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