Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis

S. O.Abd Algaffar, A. Verbon, W. W.J. van de Sande, S. A. Khalid*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
4 Downloads (Pure)

Abstract

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016mg/ml) followed by voriconazole (MIC50, 0.063mg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.

Original languageEnglish
Article numbere01338-20
JournalAntimicrobial Agents and Chemotherapy
Volume65
Issue number3
DOIs
Publication statusPublished - Mar 2021

Bibliographical note

Publisher Copyright:
© 2021 Abd Algaffar et al.

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