Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis

S. O.Abd Algaffar; A. Verbon; W. W.J. van de Sande; S. A. Khalid

doi:10.1128/AAC.01338-20

Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis

S. O.Abd Algaffar
, A. Verbon
, W. W.J. van de Sande
, S. A. Khalid^*

^*Corresponding author for this work

Medical Microbiology & Infectious Diseases

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

61 Downloads (Pure)

Abstract

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016mg/ml) followed by voriconazole (MIC50, 0.063mg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.

Original language	English
Article number	e01338-20
Journal	Antimicrobial Agents and Chemotherapy
Volume	65
Issue number	3
DOIs	https://doi.org/10.1128/AAC.01338-20
Publication status	Published - Mar 2021

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Publisher Copyright:
© 2021 Abd Algaffar et al.

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Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatisFinal published version, 434 KBLicence: CC BY

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title = "Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis",

abstract = "We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016mg/ml) followed by voriconazole (MIC50, 0.063mg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective. ",

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doi = "10.1128/AAC.01338-20",

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AU - Algaffar, S. O.Abd

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AU - Khalid, S. A.

PY - 2021/3

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AB - We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016mg/ml) followed by voriconazole (MIC50, 0.063mg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.

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Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis

Abstract

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