TY - JOUR
T1 - Different features of acute myeloid leukemia stem cell quantification in intensively treated patients
AU - Ngai, Lok Lam
AU - Reuvekamp, Tom
AU - Hanekamp, Diana
AU - Janssen, Fleur
AU - Marsbergen, Laura Oudshoorn van
AU - Carbaat-Ham, Jannemieke
AU - Hofland, Maaike A.M.
AU - Fayed, Mona M.H.E.
AU - Kelder, Angèle
AU - Scholten, Willemijn J.
AU - Snel, Alexander N.
AU - Bachas, Costa
AU - Tettero, Jesse M.
AU - Breems, Dimitri A.
AU - Fischer, Thomas
AU - Gjertsen, Bjørn T.
AU - Griškevičius, Laimonas
AU - Juliusson, Gunnar
AU - Van de Loosdrecht, Arjan A.
AU - Maertens, Johan A.
AU - Manz, Markus G.
AU - Pabst, Thomas
AU - Passweg, Jakob R.
AU - Porkka, Kimmo
AU - Valk, Peter J.M.
AU - Gradowska, Patrycja
AU - Löwenberg, Bob
AU - Ossenkoppele, Gert J.
AU - De Leeuw, David C.
AU - Cloos, Jacqueline
N1 - Publisher Copyright:
© 2025 Ferrata Storti Foundation Published under a CC BY-NC license
PY - 2025/12
Y1 - 2025/12
N2 - In acute myeloid leukemia, the burden of CD34+CD38- leukemia stem cells (LSC) has prognostic value at diagnosis and after induction chemotherapy. Since different methods of LSC quantification have been proposed, we determined the prognostic value on overall survival and incidence of relapse of these methods across European LeukemiaNet (ELN) 2017 risk groups, using data from the HOVON-SAKK132 trial. In addition, we evaluated the optimal number of acquired white blood cells for accurate LSC detection and the prognostic value of individual LSC markers. Results show that it is essential to acquire at least 1x106 white blood cells in order to assess LSC-negativity accurately. Among various LSC markers, CD44 overexpression on CD34+CD38- cells was the only marker that was not statistically significant in our panel. Testing the impact of several published variations of LSC analysis on prognostic value for overall survival and cumulative incidence of relapse showed only marginal differences, demonstrating the robust prognostic value of LSC burden. For further clinical implementation, the optimal LSC assessment may differ among ELN risk groups. In conclusion, LSC burden is a robust prognostic factor and insight into the different methods of LSC definition can facilitate the clinical implementation. Trial registration EudraCT Number: 2013-002843-26.
AB - In acute myeloid leukemia, the burden of CD34+CD38- leukemia stem cells (LSC) has prognostic value at diagnosis and after induction chemotherapy. Since different methods of LSC quantification have been proposed, we determined the prognostic value on overall survival and incidence of relapse of these methods across European LeukemiaNet (ELN) 2017 risk groups, using data from the HOVON-SAKK132 trial. In addition, we evaluated the optimal number of acquired white blood cells for accurate LSC detection and the prognostic value of individual LSC markers. Results show that it is essential to acquire at least 1x106 white blood cells in order to assess LSC-negativity accurately. Among various LSC markers, CD44 overexpression on CD34+CD38- cells was the only marker that was not statistically significant in our panel. Testing the impact of several published variations of LSC analysis on prognostic value for overall survival and cumulative incidence of relapse showed only marginal differences, demonstrating the robust prognostic value of LSC burden. For further clinical implementation, the optimal LSC assessment may differ among ELN risk groups. In conclusion, LSC burden is a robust prognostic factor and insight into the different methods of LSC definition can facilitate the clinical implementation. Trial registration EudraCT Number: 2013-002843-26.
UR - https://www.scopus.com/pages/publications/105023544009
U2 - 10.3324/haematol.2024.287090
DO - 10.3324/haematol.2024.287090
M3 - Article
C2 - 40304060
AN - SCOPUS:105023544009
SN - 0390-6078
VL - 110
SP - 2942
EP - 2953
JO - Haematologica
JF - Haematologica
IS - 12
ER -