TY - JOUR
T1 - Differential expression of estrogen receptors α and β mRNA during differentiation of human osteoblast SV-HFO cells
AU - Arts, Janine
AU - Kuiper, George G.J.M.
AU - Janssen, Josine M.M.F.
AU - Gustafsson, Jan Åke
AU - Löwik, Clemens W.G.M.
AU - Pols, Huibert A.P.
AU - Van Leeuwen, Johannes P.T.M.
PY - 1997/11/1
Y1 - 1997/11/1
N2 - Estrogens have been shown to be essential for maintaining a sufficiently high bone mineral density and ERα expression has been demonstrated in bone cells. Recently, a novel estrogen receptor, estrogen receptor β (ERβ) has been identified. Here we demonstrate that also ERβ is expressed in human osteoblasts, and that ERα and ERβ are differentially expressed during human osteoblast differentiation. ERβ mRNA expression increased gradually during osteoblast culture, resulting in an average increase of 9.9±5.3 fold (mean±S.D., n=3) at day 21 (mineralization phase) as compared to day 6 (proliferation phase). In contrast, ERα mRNA expression levels increased only slightly until day 10 (2.3±1.7 fold) and than remained constant. The observed differential regulation of ERα and β is suggestive for an additional functional role of ERβ to ERα in bone metabolism.
AB - Estrogens have been shown to be essential for maintaining a sufficiently high bone mineral density and ERα expression has been demonstrated in bone cells. Recently, a novel estrogen receptor, estrogen receptor β (ERβ) has been identified. Here we demonstrate that also ERβ is expressed in human osteoblasts, and that ERα and ERβ are differentially expressed during human osteoblast differentiation. ERβ mRNA expression increased gradually during osteoblast culture, resulting in an average increase of 9.9±5.3 fold (mean±S.D., n=3) at day 21 (mineralization phase) as compared to day 6 (proliferation phase). In contrast, ERα mRNA expression levels increased only slightly until day 10 (2.3±1.7 fold) and than remained constant. The observed differential regulation of ERα and β is suggestive for an additional functional role of ERβ to ERα in bone metabolism.
UR - http://www.scopus.com/inward/record.url?scp=0030729211&partnerID=8YFLogxK
U2 - 10.1210/endo.138.11.5652
DO - 10.1210/endo.138.11.5652
M3 - Article
C2 - 9348242
AN - SCOPUS:0030729211
SN - 0013-7227
VL - 138
SP - 5067
EP - 5070
JO - Endocrinology
JF - Endocrinology
IS - 11
ER -