Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors

Gerrit Cornelis Beekhof, Catarina Osório, Joshua J. White, Scott Van Zoomeren, Hannah van der Stok, Bilian Xiong, Ingo H.M.S. Nettersheim, Willem Ashwin Mak, Marit Runge, Francesca Romana Fiocchi, Henk Jan Boele, Freek E. Hoebeek, Martijn Schonewille*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)
28 Downloads (Pure)

Abstract

Distinct populations of Purkinje cells (PCs) with unique molecular and connectivity features are at the core of the modular organization of the cerebellum. Previously, we showed that firing activity of PCs differs between ZebrinII-positive and ZebrinII-negative cerebellar modules (Zhou et al., 2014; Wu et al., 2019). Here, we investigate the timing and extent of PC differentiation during development in mice. We found that several features of PCs, including activity levels, dendritic arborization, axonal shape and climbing fiber input, develop differentially between nodular and anterior PC populations. Although all PCs show a particularly rapid development in the second postnatal week, anterior PCs typically have a prolonged physiological and dendritic maturation. In line herewith, younger mice exhibit attenuated anterior-dependent eyeblink conditioning, but faster nodular-dependent compensatory eye movement adaptation. Our results indicate that specific cerebellar regions have unique developmental timelines which match with their related, specific forms of cerebellum-dependent behaviors.

Original languageEnglish
Article numbere63668
JournaleLife
Volume10
DOIs
Publication statusPublished - 11 Jun 2021

Bibliographical note

Funding Information:
The authors kindly thank Laura Post, Sander Kruithof and Erika Sabel-Goedknegt for excellent technical assistance; Dick Jaarsma and Lynette Lim for discussions and comments on the manuscript. This work was supported by an ERC starter grant (ERC-Stg #680235; MS), Dutch Organization for Life Sciences (Off-Road fellowship; ZonMW-451001027; CO), VENI fellowship (NWO-ENW; JJW), VIDI grant (NWO-ENW; #016.121.346, FEH) and Medical Sciences (TOP-GO #91210067, FEH) and the CJ Vaillant Fund (FEH).

Funding Information:
H2020 European Research Council ERC-Stg #680235 Martijn Schonewille ZonMw ZonMW-451001027 Catarina Os?rio Nederlandse Organisatie voor Wetenschappelijk Onderzoek #016.121.346 Freek E Hoebeek ZonMw #91210067 Freek E Hoebeek Nederlandse Organisatie voor Wetenschappelijk Onderzoek 016.Veni.192.270 Joshua J White.

Publisher Copyright:
© Beekhof et al.

Fingerprint

Dive into the research topics of 'Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors'. Together they form a unique fingerprint.

Cite this