TY - JOUR
T1 - Diffuse reflectance spectroscopy
T2 - Toward real-time quantification of steatosis in liver
AU - Evers, Daniel J.
AU - Westerkamp, Andrie C.
AU - Spliethoff, Jarich W.
AU - Pully, Vishnu V.
AU - Hompes, Daphne
AU - Hendriks, Benno H.W.
AU - Prevoo, Warner
AU - Van Velthuysen, Marie Louise F.
AU - Porte, Robert J.
AU - Ruers, Theo J.M.
N1 - Publisher Copyright:
© 2015 Steunstichting ESOT.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Summary Assessment of fatty liver grafts during orthotopic liver transplantation is a challenge due to the lack of real-time analysis options during surgery. Diffuse reflectance spectroscopy (DRS) could be a new diagnostic tool to quickly assess steatosis. Eight hundred and seventy-eight optical measurements were performed in vivo in 17 patients in liver tissue during surgery and ex vivo on liver resection specimens from 41 patients. Liver steatosis was quantified from the collected optical spectra and compared with the histology analysis from the measurement location by three independent pathologists. Twenty two patients were diagnosed with <5% steatosis, 15 patients had mild steatosis, and four had moderate steatosis. Severe steatosis was not identified. Intraclass correlation between the pathologists analysis was 0.949. A correlation of 0.854 was found between the histology and DRS analyses of liver steatosis ex vivo. For the same liver tissue, a correlation of 0.925 was demonstrated between in vivo and ex vivo DRS analysis for steatosis quantification. DRS can quantify steatosis in liver tissue both in vivo and ex vivo with good agreement compared to histopathology analysis. This analysis can be performed real time and may therefore be useful for fast objective assessment of liver steatosis in liver surgery.
AB - Summary Assessment of fatty liver grafts during orthotopic liver transplantation is a challenge due to the lack of real-time analysis options during surgery. Diffuse reflectance spectroscopy (DRS) could be a new diagnostic tool to quickly assess steatosis. Eight hundred and seventy-eight optical measurements were performed in vivo in 17 patients in liver tissue during surgery and ex vivo on liver resection specimens from 41 patients. Liver steatosis was quantified from the collected optical spectra and compared with the histology analysis from the measurement location by three independent pathologists. Twenty two patients were diagnosed with <5% steatosis, 15 patients had mild steatosis, and four had moderate steatosis. Severe steatosis was not identified. Intraclass correlation between the pathologists analysis was 0.949. A correlation of 0.854 was found between the histology and DRS analyses of liver steatosis ex vivo. For the same liver tissue, a correlation of 0.925 was demonstrated between in vivo and ex vivo DRS analysis for steatosis quantification. DRS can quantify steatosis in liver tissue both in vivo and ex vivo with good agreement compared to histopathology analysis. This analysis can be performed real time and may therefore be useful for fast objective assessment of liver steatosis in liver surgery.
UR - http://www.scopus.com/inward/record.url?scp=84926419616&partnerID=8YFLogxK
U2 - 10.1111/tri.12517
DO - 10.1111/tri.12517
M3 - Article
C2 - 25556291
AN - SCOPUS:84926419616
SN - 0934-0874
VL - 28
SP - 465
EP - 474
JO - Transplant International
JF - Transplant International
IS - 4
ER -