DNA binding properties of the integrase proteins of human immunodeficiency viruses types 1 and 2

Dik C. Van Gent, Ype Elgersma, Marian W.J. Bolk, Cornelis Vink, Ronald H.A. Plasterk*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

102 Citations (Scopus)
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Abstract

Integration of retroviral DNA into the host chromosome requires the integrase protein (IN). We overexpressed the IN proteins of human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2) in E. coli and purified them. Both proteins were found to specifically cut two nucleotides off the ends of linear viral DNA, and to integrate viral DNA into target DNA. This demonstrates that HIV IN is the only protein required for integration of HIV DNA. Although the two types of IN proteins have only 53% amino acid sequence similarity, they act with equal efficiency on both type 1 and type 2 viral DNA. Binding of IN to DNA was tested: purified IN does not bind very specifically to viral DNA ends. Nevertheless, only viral DNA ends are cleaved and integrated. We interpret this as follows: in vitro quick aspecific binding to DNA is followed by slow specific cutting and integration. IN can not find viral DNA ends in the presence of an excess of aspecific DNA; in vivo this is not required since the IN protein is in constant proximity of viral DNA in the viral core particle.

Original languageEnglish
Pages (from-to)3821-3827
Number of pages7
JournalNucleic Acids Research
Volume19
Issue number14
DOIs
Publication statusPublished - 25 Jul 1991
Externally publishedYes

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