Dual electrospinning with sacrificial fibers for engineered porosity and enhancement of tissue ingrowth

Jason Voorneveld, Anel Oosthuysen, Thomas Franz, Peter Zilla, Deon Bezuidenhout*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

Porosity, pore size and pore interconnectivity are critical factors for cellular infiltration into electrospun scaffolds. This study utilized dual electrospinning with sacrificial fiber extraction to produce scaffolds with engineered porosity and mechanical properties. Subsequently, scaffolds were covalently grafted with heparin, a known anti-coagulant with growth-factor binding properties. We hypothesized that the tissue ingrowth would correlate positively with the porosity of the scaffolds. Pellethane® (PU) was spun simultaneously with poly(ethylene oxide) (PEO, subsequently extracted). Low, medium and high porosity scaffolds and heparinized versions of each were characterized and implanted in vivo for evaluation of cellular infiltration and inflammation subcutaneously in male Wistar rats (7,14 and 28 days, n = 6). Average pore-size for low (76 ± 0.2%), medium (83 ± 0.5%) and high (90 ± 1.0%) porosity scaffolds was 4.0 ± 2.3 µm, 9.9 ± 4.2 µm and 11.1 ± 5.5 µm (p 
Original languageEnglish
Pages (from-to)1559-1572
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume105
Issue number6
Early online date29 Apr 2016
DOIs
Publication statusPublished - Aug 2017
Externally publishedYes

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