Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development

Ralph Stadhouders; S Thongjuea; C Andrieu-Soler; Robert-jan Palstra; JC Bryne; anita Heuvel; M (Martijn) Stevens; Ernie Boer; Christel Kockx; A van der Sloot; M van den Hout; Wilfred van Ijcken; D Eick; B Lenhard; Frank Grosveld; Eric Soler

doi:10.1038/emboj.2011.450

Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development

Ralph Stadhouders, S Thongjuea, C Andrieu-Soler, Robert-jan Palstra, JC Bryne, anita Heuvel, M (Martijn) Stevens, Ernie Boer, Christel Kockx, A van der Sloot, M van den Hout, Wilfred van Ijcken, D Eick, B Lenhard, Frank Grosveld, Eric Soler

Research output: Contribution to journal › Article › Academic › peer-review

109 Citations (Scopus)

Abstract

The key haematopoietic regulator Myb is essential for coordinating proliferation and differentiation. ChIP-Sequencing and Chromosome Conformation Capture (3C)-Sequencing were used to characterize the structural and protein-binding dynamics of the Myb locus during erythroid differentiation. In proliferating cells expressing Myb, enhancers within the Myb-Hbs1l intergenic region were shown to form an active chromatin hub (ACH) containing the Myb promoter and first intron. This first intron was found to harbour the transition site from transcription initiation to elongation, which takes place around a conserved CTCF site. Upon erythroid differentiation, Myb expression is downregulated and the ACH destabilized. We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping. The EMBO Journal (2012) 31, 986-999. doi: 10.1038/emboj.2011.450; Published online 13 December 2011

Original language	Undefined/Unknown
Pages (from-to)	986-999
Number of pages	14
Journal	EMBO Journal
Volume	31
Issue number	4
DOIs	https://doi.org/10.1038/emboj.2011.450
Publication status	Published - 2012

Access to Document

10.1038/emboj.2011.450

http://hdl.handle.net/1765/68644

Cite this

Stadhouders, R., Thongjuea, S., Andrieu-Soler, C., Palstra, R., Bryne, JC., Heuvel, A., Stevens, M., Boer, E., Kockx, C., van der Sloot, A., van den Hout, M., van Ijcken, W., Eick, D., Lenhard, B., Grosveld, F., & Soler, E. (2012). Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development. EMBO Journal, 31(4), 986-999. https://doi.org/10.1038/emboj.2011.450

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title = "Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development",

abstract = "The key haematopoietic regulator Myb is essential for coordinating proliferation and differentiation. ChIP-Sequencing and Chromosome Conformation Capture (3C)-Sequencing were used to characterize the structural and protein-binding dynamics of the Myb locus during erythroid differentiation. In proliferating cells expressing Myb, enhancers within the Myb-Hbs1l intergenic region were shown to form an active chromatin hub (ACH) containing the Myb promoter and first intron. This first intron was found to harbour the transition site from transcription initiation to elongation, which takes place around a conserved CTCF site. Upon erythroid differentiation, Myb expression is downregulated and the ACH destabilized. We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping. The EMBO Journal (2012) 31, 986-999. doi: 10.1038/emboj.2011.450; Published online 13 December 2011",

author = "Ralph Stadhouders and S Thongjuea and C Andrieu-Soler and Robert-jan Palstra and JC Bryne and anita Heuvel and Stevens, {M (Martijn)} and Ernie Boer and Christel Kockx and {van der Sloot}, A and {van den Hout}, M and {van Ijcken}, Wilfred and D Eick and B Lenhard and Frank Grosveld and Eric Soler",

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Stadhouders, R, Thongjuea, S, Andrieu-Soler, C, Palstra, R, Bryne, JC, Heuvel, A, Stevens, M, Boer, E, Kockx, C, van der Sloot, A, van den Hout, M, van Ijcken, W, Eick, D, Lenhard, B, Grosveld, F & Soler, E 2012, 'Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development', EMBO Journal, vol. 31, no. 4, pp. 986-999. https://doi.org/10.1038/emboj.2011.450

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T1 - Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development

AU - Stadhouders, Ralph

AU - Thongjuea, S

AU - Andrieu-Soler, C

AU - Palstra, Robert-jan

AU - Bryne, JC

AU - Heuvel, anita

AU - Stevens, M (Martijn)

AU - Boer, Ernie

AU - Kockx, Christel

AU - van der Sloot, A

AU - van den Hout, M

AU - van Ijcken, Wilfred

AU - Eick, D

AU - Lenhard, B

AU - Grosveld, Frank

AU - Soler, Eric

PY - 2012

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AB - The key haematopoietic regulator Myb is essential for coordinating proliferation and differentiation. ChIP-Sequencing and Chromosome Conformation Capture (3C)-Sequencing were used to characterize the structural and protein-binding dynamics of the Myb locus during erythroid differentiation. In proliferating cells expressing Myb, enhancers within the Myb-Hbs1l intergenic region were shown to form an active chromatin hub (ACH) containing the Myb promoter and first intron. This first intron was found to harbour the transition site from transcription initiation to elongation, which takes place around a conserved CTCF site. Upon erythroid differentiation, Myb expression is downregulated and the ACH destabilized. We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping. The EMBO Journal (2012) 31, 986-999. doi: 10.1038/emboj.2011.450; Published online 13 December 2011

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