TY - JOUR
T1 - Effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblast cell line
AU - Van Leeuwen, J. P.T.M.
AU - Bos, M. P.
AU - Lowik, C. W.G.M.
AU - Herrmann-Erlee, M. P.M.
PY - 1988
Y1 - 1988
N2 - In the present study the effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblastic cell line (UMR 106-01) is examined. In these cells the intact hormone, bPTH(1-84) and three fragments (bPTH(1-34), hPTH(1-34) and bPTH(3-34)) induce an initial increase of the ionized calcium concentration ([Ca2+](i)). The increase of the [Ca2+](i) due to bPTH(1-84) is more pronounced and of longer duration compared to the increase due to PTH fragments. For two parathyroid hormone fragments with a modified amino acid sequence ([Nle-8,Nle-18,Tyr-34]bPTH(1-34), [Nle-8,Nle-18,Tyr-34]bPTH(3-34)), no effect was found. Our observations suggest: (1) a second messenger role for intracellular ionized calcium in the action of bPTH(1-84)in osteoblasts; (2) a functional role for the COOH-terminal part in the action of bPTH(1-84); and (3) the existence of a specific part of the hormone being responsible for the increase of the intracellular ionized calcium concentration.
AB - In the present study the effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblastic cell line (UMR 106-01) is examined. In these cells the intact hormone, bPTH(1-84) and three fragments (bPTH(1-34), hPTH(1-34) and bPTH(3-34)) induce an initial increase of the ionized calcium concentration ([Ca2+](i)). The increase of the [Ca2+](i) due to bPTH(1-84) is more pronounced and of longer duration compared to the increase due to PTH fragments. For two parathyroid hormone fragments with a modified amino acid sequence ([Nle-8,Nle-18,Tyr-34]bPTH(1-34), [Nle-8,Nle-18,Tyr-34]bPTH(3-34)), no effect was found. Our observations suggest: (1) a second messenger role for intracellular ionized calcium in the action of bPTH(1-84)in osteoblasts; (2) a functional role for the COOH-terminal part in the action of bPTH(1-84); and (3) the existence of a specific part of the hormone being responsible for the increase of the intracellular ionized calcium concentration.
UR - http://www.scopus.com/inward/record.url?scp=0023896382&partnerID=8YFLogxK
M3 - Article
C2 - 3142533
AN - SCOPUS:0023896382
SN - 0169-6009
VL - 4
SP - 177
EP - 188
JO - Bone and Mineral
JF - Bone and Mineral
IS - 2
ER -