Effect of ploidy on transcription levels in cultured rat aortic smooth muscle cells

Johan W. van Neck; Patrick H.C. van Berkel; Pieter Telleman; Linda S.W. Steijns; Carla Onnekink; Henri P.J. Bloemers

doi:10.1016/0014-5793(92)80358-N

Effect of ploidy on transcription levels in cultured rat aortic smooth muscle cells

Johan W. van Neck^*, Patrick H.C. van Berkel, Pieter Telleman, Linda S.W. Steijns, Carla Onnekink, Henri P.J. Bloemers

^*Corresponding author for this work

Plastic and Reconstructive Surgery and Hand Surgery

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA expression of the genes assayed is linked with ploidy. However, when phenotypically different SMC cultures derived from newborn and adult rats were compared, transcription levels varied from gene to gene and not linked with the ploidy. Thus, differences in gene expression due to polyploidy are superimposed on those due to other phenotypical features.

Original language	English
Pages (from-to)	189-195
Number of pages	7
Journal	FEBS Letters
Volume	297
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(92)80358-N
Publication status	Published - 3 Feb 1992

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title = "Effect of ploidy on transcription levels in cultured rat aortic smooth muscle cells",

abstract = "Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA expression of the genes assayed is linked with ploidy. However, when phenotypically different SMC cultures derived from newborn and adult rats were compared, transcription levels varied from gene to gene and not linked with the ploidy. Thus, differences in gene expression due to polyploidy are superimposed on those due to other phenotypical features.",

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AU - van Berkel, Patrick H.C.

AU - Telleman, Pieter

AU - Steijns, Linda S.W.

AU - Onnekink, Carla

AU - Bloemers, Henri P.J.

PY - 1992/2/3

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N2 - Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA expression of the genes assayed is linked with ploidy. However, when phenotypically different SMC cultures derived from newborn and adult rats were compared, transcription levels varied from gene to gene and not linked with the ploidy. Thus, differences in gene expression due to polyploidy are superimposed on those due to other phenotypical features.

AB - Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA expression of the genes assayed is linked with ploidy. However, when phenotypically different SMC cultures derived from newborn and adult rats were compared, transcription levels varied from gene to gene and not linked with the ploidy. Thus, differences in gene expression due to polyploidy are superimposed on those due to other phenotypical features.

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Effect of ploidy on transcription levels in cultured rat aortic smooth muscle cells

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