TY - JOUR
T1 - Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair
T2 - In Vitro, Ex Vivo and In Vivo Evaluation
AU - Xu, Jietao
AU - Fahmy-Garcia, Shorouk
AU - Wesdorp, Marinus A.
AU - Kops, Nicole
AU - Forte, Lucia
AU - De Luca, Claudio
AU - Misciagna, Massimiliano Maraglino
AU - Dolcini, Laura
AU - Filardo, Giuseppe
AU - Labberté, Margot
AU - Vancíková, Karin
AU - Kok, Joeri
AU - van Rietbergen, Bert
AU - Nickel, Joachim
AU - Farrell, Eric
AU - Brama, Pieter A.J.
AU - van Osch, Gerjo J.V.M.
N1 - Funding Information:
This research was conducted in the EuroNanoMed III Project under the European Union’s Horizon 2020 research and innovation programme (reference number: EURONANOMED2017-077; grant agreement no 723770) with funding provided by the Applied and Engineering Sciences section of the Dutch Research Council, The Netherlands (NWO-TTW), the Science Foundation of Ireland (Grant Number SFI/16/ENM-ERA/3458), and the Ministero della Salute (IMH), Italy.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/2/16
Y1 - 2023/2/16
N2 - Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects.
AB - Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects.
UR - http://www.scopus.com/inward/record.url?scp=85148867852&partnerID=8YFLogxK
U2 - 10.3390/jfb14020111
DO - 10.3390/jfb14020111
M3 - Article
C2 - 36826910
AN - SCOPUS:85148867852
SN - 2079-4983
VL - 14
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
IS - 2
M1 - 111
ER -