TY - JOUR
T1 - Effects of different ventilatory settings on alveolar and pulmonary microvessel dimensions in pigs
AU - Damiani, Elisa
AU - Casarotta, Erika
AU - Di Bella, Caterina
AU - Galosi, Margherita
AU - Angorini, Alessio
AU - Serino, Federica
AU - Tambella, Adolfo Maria
AU - Laus, Fulvio
AU - Zuccari, Samuele
AU - Salvucci Salice, Alessio
AU - Domizi, Roberta
AU - Carsetti, Andrea
AU - Ince, Can
AU - Donati, Abele
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12/5
Y1 - 2024/12/5
N2 - Mechanical ventilation with high tidal volume (TV) or positive end-expiratory pressure (PEEP) may induce lung overinflation and increased pulmonary vascular resistance to flow. In 8 healthy mechanically ventilated pigs, we evaluated whether incident dark field (IDF) vital microscopy, applied through a small thoracotomy, could be used to evaluate changes in alveolar and pulmonary microvessel dimensions under different ventilator settings. High TV (12 ml/kg) increased alveolar diameters (from 99 ± 13 to 114 ± 6 μm, p < 0.05 repeated measures one way analysis of variance) and reduced septal capillary diameters (from 12.1 ± 1.7 to 10.5 ± 1.4 μm, p < 0.001) as compared to 8 ml/kg TV. This effect was more pronounced in non-dependent lung. Alveolar and microvessel diameters did not change with high PEEP (12 cmH2O Vs. 5 cmH2O). High FiO2 (100%) led to pulmonary vasodilation (from 12.1 ± 1.7 to 14.7 ± 1.4 μm, p < 0.001), with no change in alveolar dimensions as compared to 50% FiO2. In conclusion, IDF imaging enabled to obtain high-quality images of subpleural alveoli and microvessels. High TV ventilation may induce alveolar distension with compression of septal capillaries, thus potentially increasing dead space ventilation.
AB - Mechanical ventilation with high tidal volume (TV) or positive end-expiratory pressure (PEEP) may induce lung overinflation and increased pulmonary vascular resistance to flow. In 8 healthy mechanically ventilated pigs, we evaluated whether incident dark field (IDF) vital microscopy, applied through a small thoracotomy, could be used to evaluate changes in alveolar and pulmonary microvessel dimensions under different ventilator settings. High TV (12 ml/kg) increased alveolar diameters (from 99 ± 13 to 114 ± 6 μm, p < 0.05 repeated measures one way analysis of variance) and reduced septal capillary diameters (from 12.1 ± 1.7 to 10.5 ± 1.4 μm, p < 0.001) as compared to 8 ml/kg TV. This effect was more pronounced in non-dependent lung. Alveolar and microvessel diameters did not change with high PEEP (12 cmH2O Vs. 5 cmH2O). High FiO2 (100%) led to pulmonary vasodilation (from 12.1 ± 1.7 to 14.7 ± 1.4 μm, p < 0.001), with no change in alveolar dimensions as compared to 50% FiO2. In conclusion, IDF imaging enabled to obtain high-quality images of subpleural alveoli and microvessels. High TV ventilation may induce alveolar distension with compression of septal capillaries, thus potentially increasing dead space ventilation.
UR - http://www.scopus.com/inward/record.url?scp=85211633106&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-82244-7
DO - 10.1038/s41598-024-82244-7
M3 - Article
C2 - 39639087
AN - SCOPUS:85211633106
SN - 2045-2322
VL - 14
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 30391
ER -