Effects of Intra- and Post-Operative Ischemia on the Metabolic Profile of Clinical Liver Tissue Specimens Monitored by NMR

S Cacciatore, XY Hu, C Viertler, Marcel Kap, GA Bernhardt, HJ Mischinger, Peter Riegman, K Zatloukal, C Luchinat, P Turano

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Metabolomic profiles of tissues could greatly contribute to advancements in personalized medicine but are influenced by differences in adopted preanalytical procedures; nonhomogeneous pre- and post-excision ischemia times are potential sources of variability. In this study, we monitored the impact of ischemia on the metabolic profiles, acquired with high-resolution magic-angle-spinning H-1 NMR, of 162 human liver samples collected during and up to 6 h after routine surgery. The profiles changed significantly as a function of intraoperative warm ischemia (WI) and postresection cold ischemia (CI) time, with significant variations in the concentration of the same 16 metabolites. Therefore, a tight control of the preanalytical phase is essential for reliable metabolomic analyses of liver diseases. The NMR profiles provide a reliable "fingerprint" of ischemia and have predictive value: the best-performing predictive models are found to discriminate extreme time points of CI (0' vs 360') in the training set with cross-validation accuracy of similar to 90%; samples in the validation cohort can discriminate short (<= 60') from long (>= 180') CI with an accuracy of similar to 80%. For WI, the corresponding figures are 95.6 and 92%, respectively. Therefore, ischemia NMR profiles might become a tool for tissue quality control in biobanks.
Original languageUndefined/Unknown
Pages (from-to)5723-5729
Number of pages7
JournalJournal of Proteome Research
Issue number12
Publication statusPublished - 2013

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  • EMC MM-03-24-01

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