TY - JOUR
T1 - Effects of iron oxide incorporation for long term cell tracking on MSC differentiation in vitro and in vivo
AU - Farrell, Eric
AU - Wielopolski, Piotr
AU - Pavljasevic, P (Predrag)
AU - van Tiel, Sandra
AU - Jahr, Holger
AU - Verhaar, Jan
AU - Weinans, HH
AU - Krestin, Gabriel
AU - O'Brien, FJ
AU - van Osch, Gerjo
AU - Bernsen, Monique
PY - 2008
Y1 - 2008
N2 - Successful cell therapy will depend on the ability to monitor transplanted cells. With cell labeling, it is important to demonstrate efficient long term labeling without deleterious effects on cell phenotype and differentiation capacity. We demonstrate long term (7 weeks) retention of superparamagnetic iron oxide particles (SPIO) by mesenchymal stem cells (MSCs) in vivo, detectable by MRI. In vitro, multilineage differentiation (osteogenic, chondrogenic and adipogenic) was demonstrated by histological evaluation and molecular analysis in SPIO labeled and unlabeled cells. Gene expression levels were comaparable to unlabeled controls in adipogenic and chondrogenic conditions however not in the osteogenic condition. MSCs seeded into a scaffold for 21 days and implanted subcutaneously into nude mice for 4 weeks, showed profoundly altered phenotypes in SPIO labeled samples compared to implanted unlabeled control scaffolds, indicating chondrogenic differentiation. This study demonstrates long term MSC traceability using SPIO and MRI, uninhibited multilineage MSC differentiation following SPIO labeling, though with subtle but significant phenotypical alterations. (C) 2008 Elsevier Inc. All rights reserved.
AB - Successful cell therapy will depend on the ability to monitor transplanted cells. With cell labeling, it is important to demonstrate efficient long term labeling without deleterious effects on cell phenotype and differentiation capacity. We demonstrate long term (7 weeks) retention of superparamagnetic iron oxide particles (SPIO) by mesenchymal stem cells (MSCs) in vivo, detectable by MRI. In vitro, multilineage differentiation (osteogenic, chondrogenic and adipogenic) was demonstrated by histological evaluation and molecular analysis in SPIO labeled and unlabeled cells. Gene expression levels were comaparable to unlabeled controls in adipogenic and chondrogenic conditions however not in the osteogenic condition. MSCs seeded into a scaffold for 21 days and implanted subcutaneously into nude mice for 4 weeks, showed profoundly altered phenotypes in SPIO labeled samples compared to implanted unlabeled control scaffolds, indicating chondrogenic differentiation. This study demonstrates long term MSC traceability using SPIO and MRI, uninhibited multilineage MSC differentiation following SPIO labeling, though with subtle but significant phenotypical alterations. (C) 2008 Elsevier Inc. All rights reserved.
U2 - 10.1016/j.bbrc.2008.02.159
DO - 10.1016/j.bbrc.2008.02.159
M3 - Article
C2 - 18336785
SN - 0006-291X
VL - 369
SP - 1076
EP - 1081
JO - Biochemical & Biophysical Research Communications
JF - Biochemical & Biophysical Research Communications
IS - 4
ER -