Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

C E G Havenith, J M van Haarst, A J Breedijk, M G Betjes, H C Hoogsteden, R H J Beelen*, E C Hoefsmit

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)
10 Downloads (Pure)

Abstract

In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.

Original languageEnglish
Pages (from-to)339-43
Number of pages5
JournalClinical and Experimental Immunology
Volume96
Issue number2
DOIs
Publication statusPublished - May 1994

Fingerprint

Dive into the research topics of 'Enrichment and characterization of dendritic cells from human bronchoalveolar lavages'. Together they form a unique fingerprint.

Cite this