Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

C E G Havenith; J M van Haarst; A J Breedijk; M G Betjes; H C Hoogsteden; R H J Beelen; E C Hoefsmit

doi:10.1111/j.1365-2249.1994.tb06564.x

Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

C E G Havenith, J M van Haarst, A J Breedijk, M G Betjes, H C Hoogsteden, R H J Beelen^*, E C Hoefsmit

^*Corresponding author for this work

Immunology

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Abstract

In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.

Original language	English
Pages (from-to)	339-43
Number of pages	5
Journal	Clinical and Experimental Immunology
Volume	96
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2249.1994.tb06564.x
Publication status	Published - May 1994

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title = "Enrichment and characterization of dendritic cells from human bronchoalveolar lavages",

abstract = "In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.",

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AU - Havenith, C E G

AU - van Haarst, J M

AU - Breedijk, A J

AU - Betjes, M G

AU - Hoogsteden, H C

AU - Beelen, R H J

AU - Hoefsmit, E C

PY - 1994/5

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N2 - In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.

AB - In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.

U2 - 10.1111/j.1365-2249.1994.tb06564.x

DO - 10.1111/j.1365-2249.1994.tb06564.x

M3 - Article

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SN - 0009-9104

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SP - 339

EP - 343

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

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ER -

Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

Abstract

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