Establishment of human malignant mesothelioma cell lines

Marjan A. Versnel; Monique J. Bouts; Henk C. Hoogsteden; Theodorus H.Der Van Kwast; Mick Delahaye; Anne Hagemeijer

doi:10.1002/ijc.2910440212

Establishment of human malignant mesothelioma cell lines

Marjan A. Versnel^*
, Monique J. Bouts
, Henk C. Hoogsteden
, Theodorus H.Der Van Kwast
, Mick Delahaye
, Anne Hagemeijer

^*Corresponding author for this work

Erasmus University Rotterdam
University Hospital Dijkzigt

Research output: Contribution to journal › Article › Academic › peer-review

53 Citations (Scopus)

Abstract

Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.

Original language	English
Pages (from-to)	256-260
Number of pages	5
Journal	International Journal of Cancer
Volume	44
Issue number	2
DOIs	https://doi.org/10.1002/ijc.2910440212
Publication status	Published - 15 Aug 1989

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abstract = "Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.",

author = "Versnel, \{Marjan A.\} and Bouts, \{Monique J.\} and Hoogsteden, \{Henk C.\} and \{Van Kwast\}, \{Theodorus H.Der\} and Mick Delahaye and Anne Hagemeijer",

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AU - Versnel, Marjan A.

AU - Bouts, Monique J.

AU - Hoogsteden, Henk C.

AU - Van Kwast, Theodorus H.Der

AU - Delahaye, Mick

AU - Hagemeijer, Anne

PY - 1989/8/15

Y1 - 1989/8/15

N2 - Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.

AB - Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.

UR - https://www.scopus.com/pages/publications/0024464096

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DO - 10.1002/ijc.2910440212

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VL - 44

SP - 256

EP - 260

JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 2

ER -

Establishment of human malignant mesothelioma cell lines

Abstract

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