Ethanol stimulates expression of functional H+, K+-ATPase in Sf9 cells

Corné H.W. Klaassen, Herman G.P. Swarts, Jan Joep H.H.M. De Pont*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)


The baculovirus expression system is suitable for functional expression of gastric H+, K+-ATPase. Expression of functional H+, K+-ATPase in Sf9 cells is accompanied by synthesis of large amounts of non-functional subunits. When H+, K+-ATPase is synthesised in the presence of 150-250 mM ethanol in the culture medium, two to threefold higher levels of functional H+, K+-ATPase are produced due to the formation of more functional subunits rather than to an increase of subunits per se. The catalytical properties of the ethanol-produced H+, K+-ATPase are indistinguishable from control preparations. The mechanism by which ethanol stimulates the formation of functional H+, K+-ATPase probably involves a direct effect on the physical properties of Sf9 membranes. In addition there also might be an indirect effect through ethanol inducible stress proteins acting as molecular chaperones.

Original languageEnglish
Pages (from-to)907-913
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 1995
Externally publishedYes


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