Human bone marrow-derived mesenchymal stem/stromal cells (BM-MSC) are adult multipotent progenitor cells that can be isolated from bone marrow. BM-MSCs have the ability to be expanded and differentiated into the chondrogenic lineage in vitro. Here we describe a standardized method to expand and chondrogenically differentiate human BM-MSCs, highlighting how to overcome technical challenges and indicating the most common readout parameters to evaluate the chondrogenic differentiation capacity.
|Title of host publication||Osteoporosis and Osteoarthritis|
|Place of Publication||[S.l.]|
|Number of pages||15|
|Publication status||Published - 2021|
|Series||Methods in Molecular Biology|