Experimental colitis in mice: Effects of olsalazine on eicosanoid production in colonic tissue

F. J. Zijlstra*, I. M. Garrelds, A. P.M. van Dijk, J. H.P. Wilson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

In rodents colitis can be induced by adding 2% (w/v) carrageenan (CARR) for 4 weeks or 10% (w/v) dextran sulphate sodium (DSS) for 7 days to their drinking water. These models are suitable to test antiinflammatory drugs used in inflammatory bowel disease in man. Mice were treated with olsalazine (400 mg/kg body wt) starting 7 days before the DSS or CARR administration. Colonic tissues were incubated with [1-14C]-arachidonic acid and stimulated with A23187 and, thereafter, the pattern of eicosanoids was determined by separation on HPLC. DSS and CARR produced a marked diffuse inflammatory response in the colon and a subsequent 5-fold increase of all eicosanoids after DSS, whereas after CARR only a 2-fold increase of PGs was observed. Olsalazine treatment decreased all cyclooxygenase and lipoxygenase products to baseline levels.

Original languageEnglish
Pages (from-to)C76-C78
JournalAgents and Actions
Volume36
Issue number1 Supplement
DOIs
Publication statusPublished - Mar 1992

Fingerprint

Dive into the research topics of 'Experimental colitis in mice: Effects of olsalazine on eicosanoid production in colonic tissue'. Together they form a unique fingerprint.

Cite this