TY - JOUR
T1 - Expression of basic fibroblast growth factor and fibroblast growth factor receptor genes in cultured rat aortic smooth muscle cells
AU - van Neck, Johan W.
AU - Medina, John J.
AU - Onnekink, Carla
AU - Schwartz, Stephen M.
AU - Bloemers, Henri P.J.
PY - 1995/4/4
Y1 - 1995/4/4
N2 - Basic fibroblast growth factor (bFGF) exerts a differential effect on DNA synthesis, bFGF mRNA synthesis, and expression of FGF-receptor genes by cultured smooth muscle cells from aortae of newborn and adult rats (used as a model in atherosclerosis research). Cells from adult animals, are more sensitive to bFGF, and bFGF triggers its own mRNA synthesis. Moreover, the level of the transcript of the FGFR-1 gene (coding for the most abundant FGF-receptor in smooth muscle cells) is higher in smooth muscle cells from adult rats. In contrast, the FGFR-3 gene only is expressed in smooth muscle cells from newborn rats. Crosslinking of [125I]bFGF to its receptor showed 130 kDa and 160 kDa complexes both in newborn and adult smooth muscle cells.
AB - Basic fibroblast growth factor (bFGF) exerts a differential effect on DNA synthesis, bFGF mRNA synthesis, and expression of FGF-receptor genes by cultured smooth muscle cells from aortae of newborn and adult rats (used as a model in atherosclerosis research). Cells from adult animals, are more sensitive to bFGF, and bFGF triggers its own mRNA synthesis. Moreover, the level of the transcript of the FGFR-1 gene (coding for the most abundant FGF-receptor in smooth muscle cells) is higher in smooth muscle cells from adult rats. In contrast, the FGFR-3 gene only is expressed in smooth muscle cells from newborn rats. Crosslinking of [125I]bFGF to its receptor showed 130 kDa and 160 kDa complexes both in newborn and adult smooth muscle cells.
UR - http://www.scopus.com/inward/record.url?scp=0028965710&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(94)00247-Z
DO - 10.1016/0167-4781(94)00247-Z
M3 - Article
C2 - 7711064
AN - SCOPUS:0028965710
SN - 0167-4781
VL - 1261
SP - 210
EP - 214
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 2
ER -