Expression of basic fibroblast growth factor and fibroblast growth factor receptor genes in cultured rat aortic smooth muscle cells

Johan W. van Neck, John J. Medina, Carla Onnekink, Stephen M. Schwartz, Henri P.J. Bloemers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Basic fibroblast growth factor (bFGF) exerts a differential effect on DNA synthesis, bFGF mRNA synthesis, and expression of FGF-receptor genes by cultured smooth muscle cells from aortae of newborn and adult rats (used as a model in atherosclerosis research). Cells from adult animals, are more sensitive to bFGF, and bFGF triggers its own mRNA synthesis. Moreover, the level of the transcript of the FGFR-1 gene (coding for the most abundant FGF-receptor in smooth muscle cells) is higher in smooth muscle cells from adult rats. In contrast, the FGFR-3 gene only is expressed in smooth muscle cells from newborn rats. Crosslinking of [125I]bFGF to its receptor showed 130 kDa and 160 kDa complexes both in newborn and adult smooth muscle cells.

Original languageEnglish
Pages (from-to)210-214
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1261
Issue number2
DOIs
Publication statusPublished - 4 Apr 1995

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