Expression of lipocortins in human bronchial epithelial cells: effects of IL-1ß, TNF-α, LPS and dexamethasone

M. M. Verheggen; H. I.M. De Bont; P. W.C. Adriaansen-Soeting; B. J.A. Goense; C. J.A.M. Tak; H. C. Hoogsteden; P. T.W. Van Hal; M. A. Versnel

doi:10.1155/S0962935196000300

Expression of lipocortins in human bronchial epithelial cells: effects of IL-1ß, TNF-α, LPS and dexamethasone

M. M. Verheggen, H. I.M. De Bont, P. W.C. Adriaansen-Soeting, B. J.A. Goense, C. J.A.M. Tak, H. C. Hoogsteden, P. T.W. Van Hal, M. A. Versnel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

In this study, we investigated the expression of lipocortin I and II (annexin I and II) in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-lß, TNF-α or LPS for 24h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGFla production was significantly increased. This DL-lß- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

Original language	English
Pages (from-to)	210-217
Number of pages	8
Journal	Mediators of Inflammation
Volume	5
Issue number	3
DOIs	https://doi.org/10.1155/S0962935196000300
Publication status	Published - 1996

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title = "Expression of lipocortins in human bronchial epithelial cells: effects of IL-1{\ss}, TNF-α, LPS and dexamethasone",

abstract = "In this study, we investigated the expression of lipocortin I and II (annexin I and II) in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-l{\ss}, TNF-α or LPS for 24h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGFla production was significantly increased. This DL-l{\ss}- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.",

author = "Verheggen, {M. M.} and {De Bont}, {H. I.M.} and Adriaansen-Soeting, {P. W.C.} and Goense, {B. J.A.} and Tak, {C. J.A.M.} and Hoogsteden, {H. C.} and {Van Hal}, {P. T.W.} and Versnel, {M. A.}",

year = "1996",

doi = "10.1155/S0962935196000300",

language = "English",

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pages = "210--217",

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T1 - Expression of lipocortins in human bronchial epithelial cells

T2 - effects of IL-1ß, TNF-α, LPS and dexamethasone

AU - Verheggen, M. M.

AU - De Bont, H. I.M.

AU - Adriaansen-Soeting, P. W.C.

AU - Goense, B. J.A.

AU - Tak, C. J.A.M.

AU - Hoogsteden, H. C.

AU - Van Hal, P. T.W.

AU - Versnel, M. A.

PY - 1996

Y1 - 1996

N2 - In this study, we investigated the expression of lipocortin I and II (annexin I and II) in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-lß, TNF-α or LPS for 24h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGFla production was significantly increased. This DL-lß- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

AB - In this study, we investigated the expression of lipocortin I and II (annexin I and II) in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-lß, TNF-α or LPS for 24h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGFla production was significantly increased. This DL-lß- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

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DO - 10.1155/S0962935196000300

M3 - Article

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AN - SCOPUS:0029889007

SN - 0962-9351

VL - 5

SP - 210

EP - 217

JO - Mediators of Inflammation

JF - Mediators of Inflammation

IS - 3

ER -

Expression of lipocortins in human bronchial epithelial cells: effects of IL-1ß, TNF-α, LPS and dexamethasone

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