Ferumoxides-protamine sulfate is more effective than ferucarbotran for cell labeling: Implications for clinically applicable cell tracking using MRI

G. M. Van Buul*, E. Farrell, N. Kops, S. T. Van Tiel, P. K. Bos, H. Weinans, G. P. Krestin, G. J.V.M. Van Osch, M. R. Bernsen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

The use of superparamagnetic iron oxide (SPIO) for labeling cells holds great promise for clinically applicable cell tracking using magnetic resonance imaging. For clinical application, an effectively and specifically labeled cell preparation is highly desired (i.e. a large amount of intracellular iron and a negligible amount of extracellular iron). In this study we performed a direct comparison of two SPIO labeling strategies that have both been reported as efficient and clinically translatable approaches. These approaches are cell labeling using ferumoxides-protamine complexes or ferucarabotran particles. Cell labeling was performed on primary human bone marrow stromal cells (hBMSCs) and chondrocytes. For both cell types ferumoxides-protamine resulted in a higher percentage of labeled cells, a higher total iron load, a larger amount of intracellular iron and a lower amount of extracellular iron aggregates, compared with ferucarbotran. Consequently, hBMSC and chondrocyte labeling with ferumoxides-protamine is more effective and results in more specific cell labeling than ferucarbotran.

Original languageEnglish
Pages (from-to)230-236
Number of pages7
JournalContrast Media & Molecular Imaging
Volume4
Issue number5
DOIs
Publication statusPublished - Sept 2009

Research programs

  • EMC MUSC-01-51-01
  • EMC NIHES-03-30-03
  • EMC OR-01-62-02

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