Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

RSRM Martherus; Wim Sluiter; EDJ Timmer; SJV VanHerle; HJM Smeets; TAY Ayoubi

doi:10.1016/j.bbrc.2010.09.109

Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

RSRM Martherus, Wim Sluiter, EDJ Timmer, SJV VanHerle, HJM Smeets, TAY Ayoubi

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

32 Citations (Scopus)

Abstract

Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity. (C) 2010 Published by Elsevier Inc.

Original language	Undefined/Unknown
Pages (from-to)	203-208
Number of pages	6
Journal	Biochemical & Biophysical Research Communications
Volume	402
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.09.109
Publication status	Published - 2010

Access to Document

10.1016/j.bbrc.2010.09.109

http://hdl.handle.net/1765/27490

Cite this

@article{6027bea363a8428bb3a9eb4501af5e1d,

title = "Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association",

abstract = "Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity. (C) 2010 Published by Elsevier Inc.",

author = "RSRM Martherus and Wim Sluiter and EDJ Timmer and SJV VanHerle and HJM Smeets and TAY Ayoubi",

year = "2010",

doi = "10.1016/j.bbrc.2010.09.109",

language = "Undefined/Unknown",

volume = "402",

pages = "203--208",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

AU - Martherus, RSRM

AU - Sluiter, Wim

AU - Timmer, EDJ

AU - VanHerle, SJV

AU - Smeets, HJM

AU - Ayoubi, TAY

PY - 2010

Y1 - 2010

N2 - Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity. (C) 2010 Published by Elsevier Inc.

AB - Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity. (C) 2010 Published by Elsevier Inc.

U2 - 10.1016/j.bbrc.2010.09.109

DO - 10.1016/j.bbrc.2010.09.109

M3 - Article

SN - 0006-291X

VL - 402

SP - 203

EP - 208

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -