Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve*, Mandy van Brakel, Rebecca Wijers, Reno Debets, Dora Hammerl

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic

18 Downloads (Pure)


Prior to clinical testing of adoptive T-cell therapy with T-cell receptor (TCR)-engineered T cells, TCRs need to be retrieved, annotated, gene-transferred, and extensively tested in vitro to accurately assess specificity and sensitivity of target recognition. Here, we present a fundamental series of protocols that cover critical preclinical parameters, thereby enabling the selection of candidate TCRs for clinical testing.

Original languageEnglish
Title of host publicationImmunogenetics
Subtitle of host publicationMethods and protocols
EditorsAnton W. Langerak
Number of pages21
ISBN (Electronic)9781071621158
Publication statusPublished - 2022

Publication series

SeriesMethods in Molecular Biology

Bibliographical note

Publisher Copyright: © 2022, The Author(s).


Dive into the research topics of 'Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy'. Together they form a unique fingerprint.

Cite this