Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve*, Mandy van Brakel, Rebecca Wijers, Reno Debets, Dora Hammerl

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic

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Abstract

Prior to clinical testing of adoptive T-cell therapy with T-cell receptor (TCR)-engineered T cells, TCRs need to be retrieved, annotated, gene-transferred, and extensively tested in vitro to accurately assess specificity and sensitivity of target recognition. Here, we present a fundamental series of protocols that cover critical preclinical parameters, thereby enabling the selection of candidate TCRs for clinical testing.

Original languageEnglish
Title of host publicationImmunogenetics
Subtitle of host publicationMethods and protocols
EditorsAnton W. Langerak
Chapter13
Pages209-229
Number of pages21
ISBN (Electronic)9781071621158
DOIs
Publication statusPublished - 2022

Publication series

SeriesMethods in Molecular Biology
Volume2453
ISSN1064-3745

Bibliographical note

Publisher Copyright: © 2022, The Author(s).

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