Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve; Mandy van Brakel; Rebecca Wijers; Reno Debets; Dora Hammerl

doi:10.1007/978-1-0716-2115-8_13

Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve^*, Mandy van Brakel, Rebecca Wijers, Reno Debets, Dora Hammerl

^*Corresponding author for this work

Medical Oncology

Research output: Chapter/Conference proceeding › Chapter › Academic

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Abstract

Prior to clinical testing of adoptive T-cell therapy with T-cell receptor (TCR)-engineered T cells, TCRs need to be retrieved, annotated, gene-transferred, and extensively tested in vitro to accurately assess specificity and sensitivity of target recognition. Here, we present a fundamental series of protocols that cover critical preclinical parameters, thereby enabling the selection of candidate TCRs for clinical testing.

Original language	English
Title of host publication	Immunogenetics
Subtitle of host publication	Methods and protocols
Editors	Anton W. Langerak
Chapter	13
Pages	209-229
Number of pages	21
ISBN (Electronic)	9781071621158
DOIs	https://doi.org/10.1007/978-1-0716-2115-8_13
Publication status	E-pub ahead of print - 28 May 2022

Publication series

Series	Methods in Molecular Biology
Volume	2453
ISSN	1064-3745

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978-1-0716-2115-8_13Final published version, 468 KBLicence: CC BY

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Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

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