Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Gustavo Valbuena; Barry Rockx; Olivier Escaffre

doi:10.1007/978-1-0716-3283-3_14

Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Gustavo Valbuena, Barry Rockx, Olivier Escaffre^*

^*Corresponding author for this work

Virology

Research output: Chapter/Conference proceeding › Chapter › Academic

Abstract

The development of humanized mouse models has recently opened new avenues in the field of infectious diseases. These models allow research on many human viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (NSG) mice by the bone marrow, liver, and thymus (BLT) reconstitution method as well as the process of human lung engraftment. We then describe how to infect these human lung grafts with the paramyxovirus Nipah virus (NiV) that can cause lethal respiratory disease in humans, and for which there is only limited understanding of pathogenesis to acute lung injury.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	191-204
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-0716-3283-3_14
Publication status	Published - 2023

Publication series

Series	Methods in Molecular Biology
Volume	2682
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© 2023, Springer Science+Business Media, LLC, part of Springer Nature.

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10.1007/978-1-0716-3283-3_14

Cite this

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Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Abstract

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