Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke, Zoe Hanss, Javier Jarazo, Francois Massart, Arkadiusz Rybicki, Elizabet Petkovski, Enrico Glaab, Ibrahim Boussaad, Vincenzo Bonifati, Jens Christian Schwamborn, Wim Mandemakers, Rejko Krueger

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Abstract

We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson’s disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.
Original languageEnglish
Article number102815
JournalStem Cell Research
Volume62
DOIs
Publication statusPublished - Jul 2022

Bibliographical note

Funding Information:
The current work was supported by the Fonds National de Recherche (FNR) within the PEARL Excellence Programme [FNR/P13/6682797] to RK, and the MiRisk project [C17/BM/11676395], and by the Stichting ParkinsonFonds, The Netherlands [grant SPF-1870].

Publisher Copyright:
© 2022 The Author(s)

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