The structure and genetic organization of the transcription unit of the c-sis proto-oncogene was determined. Comparative nucleotide sequence analysis of the exon sequences of feline and human c-sis revealed a very high degree of homology. The cap site as well as the poly(A)-addition site of the sis transcript of each species was identified and found in similar positions. An insert of 4 amino acids was found In the deduced translational product of feline c-sis and It was located at the amino-terminus of the region that constituted the platelet-derived growth factor domain. An insert of 149 bp present at the 5′ end of exon 7 of human c-sis but absent In the simian sarcoma virus v-sis oncogene was also present In the feline c-sis proto-oncogene.
|Number of pages||12|
|Journal||Nucleic Acids Research|
|Publication status||Published - 11 Feb 1987|
Bibliographical noteFunding Information:
ACKNOWLEDGEMENTS We thank Adrie van Bokhoven, Inge van Renesse, Peter Hendriksen and Saskya Ebeling for their excellent technical assistance. This work was supported by the Netherlands Cancer Foundation, Contract No. NUKC Bioch. 85~9.