Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan

Anastasia P. Litvintseva*, Sahar Bakhiet, Lalitha Gade, Darlene D. Wagner, Ujwal R. Bagal, Dhwani Batra, Emily Norris, Lavanya Rishishwar, Karlyn D. Beer, Emmanuel Edwar Siddig, Najwa Adam Mhmoud, Nancy A. Chow, Ahmed Fahal

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Madurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of M. mycetomatis and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that M. mycetomatis reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +/-21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including Streptococcus sp., Staphylococcus sp. and others.

Original languageEnglish
Article numbere0010787
JournalPLoS Neglected Tropical Diseases
Volume16
Issue number11
DOIs
Publication statusPublished - 2 Nov 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 Litvintseva AP, Bakhiet S, Gade L, Wagner DD, Bagal UR, Batra D, et al.

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