Glycolytic oligodendrocytes maintain myelin and long-term axonal integrity

U Funfschilling, LM Supplie, D Mahad, S Boretius, AS Saab, J Edgar, BG Brinkmann, CM Kassmann, ID Tzvetanova, W Mobius, F Diaz, Dies Meijer, U Suter, B Hamprecht, MW Sereda, CT Moraes, J Frahm, S (Sandra) Goebbels, KA Nave

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1046 Citations (Scopus)


Oligodendrocytes, the myelin-forming glial cells of the central nervous system, maintain long-term axonal integrity(1-3). However, the underlying support mechanisms are not understood(4). Here we identify a metabolic component of axon-glia interactions by generating conditional Cox10 (protoheme IX farnesyltransferase) mutant mice, in which oligodendrocytes and Schwann cells fail to assemble stable mitochondrial cytochrome c oxidase (COX, also known as mitochondrial complex IV). In the peripheral nervous system, Cox10 conditional mutants exhibit severe neuropathy with dysmyelination, abnormal Remak bundles, muscle atrophy and paralysis. Notably, perturbing mitochondrial respiration did not cause glial cell death. In the adult central nervous system, we found no signs of demyelination, axonal degeneration or secondary inflammation. Unlike cultured oligodendrocytes, which are sensitive to COX inhibitors(5), post-myelination oligodendrocytes survive well in the absence of COX activity. More importantly, by in vivo magnetic resonance spectroscopy, brain lactate concentrations in mutants were increased compared with controls, but were detectable only in mice exposed to volatile anaesthetics. This indicates that aerobic glycolysis products derived from oligodendrocytes are rapidly metabolized within white matter tracts. Because myelinated axons can use lactate when energy-deprived(6), our findings suggest a model in which axon-glia metabolic coupling serves a physiological function.
Original languageUndefined/Unknown
Pages (from-to)517-521
Number of pages5
Issue number7399
Publication statusPublished - 2012

Research programs

  • EMC MGC-01-12-03

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