Gray matter involvement in radiologically isolated syndrome

Andrés Labiano-Fontcuberta, Virginia Mato-Abad, Juan Álvarez-Linera, Juan Antonio Hernández-Tamames, M. Luisa Martínez-Ginés, Yolanda Aladro, Lucía Ayuso, Ángela Domingo-Santos, Julián Benito-León*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)
1 Downloads (Pure)


The unanticipated magnetic resonance imaging (MRI) detection in the brain of asymptomatic subjects of white matter lesions suggestive of multiple sclerosis has recently been named as radiologically isolated syndrome (RIS). The pathophysiological processes of RIS remain largely unknown and questions as to whether gray matter alterations actually occur in this entity are yet to be investigated in more detail. By means of a 3 T multimodal MRI approach, we searched for cortical and deep gray matter changes in a cohort of RIS patients. Seventeen RIS patients, 17 clinically isolated syndrome (CIS) patients (median disease duration from symptom onset=12 months), and 17 healthy controls underwent MRI and neuropsychological testing. Normalized deep gray matter volumes and regional cortical thickness were assessed using FreeSurfer. SIENAX was used to obtain normalized global and cortical brain volumes. Voxelwise morphometry analysis was performed by using SPM8 software to localize regions of brain tissue showing significant changes of fractional anisotropy or mean diffusivity. Although no differences were observed between CIS and healthy controls groups, RIS patients showed significantly lower normalized cortical volume (673±27.07 vs 641±35.88 [cm3±103, Tukey P test=0.009) and mean thalamic volume (0.0051±0.4 vs 0.0046±0.4 mm, P=0.014) compared with healthy controls. RIS patients also showed significant thinning in a number of cortical areas, that were primarily distributed in frontal and temporal lobes (P<0.05, uncorrected). Strong correlations were observed between T2-white matter lesion volume and regional cortical thickness (rho spearman ranging from 0.60 to 0.80). Our data suggest that white matter lesions on T2-weighted images are not the only hallmark of RIS. Future longitudinal studies with larger samples are warranted to better clarify the effect of RIS-related white matter lesions on gray matter tissue.

Original languageEnglish
Pages (from-to)e3208
JournalMedicine (United States)
Issue number13
Publication statusPublished - 2016
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported by Teva neuroscience and FEDER funds. JB-L is supported by the National Institutes of Health, Bethesda, MD, USA (NINDS #R01 NS39422), the Commission of the European Union (grant ICT-2011-287739, NeuroTREMOR), the Ministry of Economy and Competitiveness (grant RTC-2015-3967-1, NetMD\-Platform for the tracking of movement disorders), and the Spanish Health Research Agency (grant FIS PI12/01602).

Publisher Copyright:
Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.


Dive into the research topics of 'Gray matter involvement in radiologically isolated syndrome'. Together they form a unique fingerprint.

Cite this