Guanine quadruplex structures localize to heterochromatin

RF Hoffmann; YM Moshkin; S Mouton; NA Grzeschik; RD Kalicharan; J Kuipers; AHG Wolters; K Nishida; Aleksandr Romashchenko; J Postberg; H Lipps; E Berezikov; OCM Sibon; BNG Giepmans; PM Lansdorp

doi:10.1093/nar/gkv900

Guanine quadruplex structures localize to heterochromatin

RF Hoffmann, YM Moshkin, S Mouton, NA Grzeschik, RD Kalicharan, J Kuipers, AHG Wolters, K Nishida, Aleksandr Romashchenko, J Postberg, H Lipps, E Berezikov, OCM Sibon, BNG Giepmans, PM Lansdorp

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

Original language	Undefined/Unknown
Pages (from-to)	152-163
Number of pages	12
Journal	Nucleic Acids Research
Volume	44
Issue number	1
DOIs	https://doi.org/10.1093/nar/gkv900
Publication status	Published - 2016

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10.1093/nar/gkv900

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title = "Guanine quadruplex structures localize to heterochromatin",

abstract = "Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.",

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T1 - Guanine quadruplex structures localize to heterochromatin

AU - Hoffmann, RF

AU - Moshkin, YM

AU - Mouton, S

AU - Grzeschik, NA

AU - Kalicharan, RD

AU - Kuipers, J

AU - Wolters, AHG

AU - Nishida, K

AU - Romashchenko, Aleksandr

AU - Postberg, J

AU - Lipps, H

AU - Berezikov, E

AU - Sibon, OCM

AU - Giepmans, BNG

AU - Lansdorp, PM

PY - 2016

Y1 - 2016

N2 - Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

AB - Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

U2 - 10.1093/nar/gkv900

DO - 10.1093/nar/gkv900

M3 - Article

SN - 0305-1048

VL - 44

SP - 152

EP - 163

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 1

ER -