Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza; H-D Chang; A Radbruch; M Akdis; I Andrae; F Annunziato; P Bacher; V Barnaba; L Battistini; WM Bauer; S Baumgart; B Becher; W Beisker; C Berek; A Blanco; G Borsellino; PE Boulais; RR Brinkman; M Buescher; DH Busch; TP Bushnell; X Cao; A Cavani; PK Chattopadhyay; Q Cheng; S Chow; M Clerici; A Cooke; A Cosma; L Cosmi; A Cumano; VD Dang; D Davies; S De Biasi; G Del Zotto; S Della Bella; P Dellabona; G Deniz; M Dessing; A Diefenbach; J Di Santo; F Dieli; A Dolf; VS Donnenberg; T Doerner; GRA Ehrhardt; E Endl; P Engel; B Engelhardt; C Esser; B Everts; A Dreher; CS Falk; TA Fehniger; A Filby; S Fillatreau; M Follo; I Foerster; J Foster; GA Foulds; PS Frenette; D Galbraith; N Garbi; M Dolores Garcia-Godoy; J Geginat; K Ghoreschi; L Gibellini; C Goettlinger; CS Goodyear; A Gori; J Grogan; M Gross; A Gruetzkau; D Grummitt; J Hahn; Q Hammer; AE Hauser; DL Haviland; D Hedley; G Herrera; M Herrmann; F Hiepe; T Holland; P Hombrink; JP Houston; BF Hoyer; B Huang; CA Hunter; A Iannone; H-M Jaeck; B Javega; S Jonjic; K Juelke; S Jung; T Kaiser; T Kalina; B Keller; Salima Khan; D Kienhoefer; T Kroneis; D Kunkel; C Kurts; P Kvistborg; J Lannigan; O Lantz; A Larbi; S LeibundGut-Landmann; MD Leipold; MK Levings; V Litwin; Y Liu; M Lohoff; G Lombardi; L Lopez; A Lovett-Racke; Erik Lubberts; B Ludewig; E Lugli; HT Maecker; G Martrus; G Matarese; C Maueroeder; M McGrath; I McInnes; HE Mei; F Melchers; S Melzer; D Mielenz; K Mills; D Mirrer; J Mjosberg; J Moore; B Moran; A Moretta; L Moretta; TR Mosmann; S Mueller; W Muller; C Munz; G Multhoff; LE Munoz; KM Murphy; T Nakayama; M Nasi; C Neudoerfl; J Nolan; S Nourshargh; J-E O'Connor; W Ouyang; A Oxenius; R Palankar; I Panse; P Peterson; C Peth; J Petriz; D Philips; W Pickl; S Piconese; M Pinti; AG Pockley; MJ Podolska; C Pucillo; SA Quataert; TRDJ Radstake; B Rajwa; JA Rebhahn; D Recktenwald; EBM Remmerswaal; K Rezvani; LG Rico; JP Robinson; C Romagnani; A Rubartelli; B Ruckert; J Ruland; S Sakaguchi; F Sala-de-Oyanguren; Y Samstag; S Sanderson; B Sawitzki; A Scheffold; M Schiemann; F Schildberg; E Schimisky; SA Schmid; S Schmitt; K Schober; T Schueler; AR Schulz; T Schumacher; C Scotta; TV Shankey; A Shemer; A-K Simon; J Spidlen; AM Stall; R Stark; C Stehle; M Stein; T Steinmetz; H Stockinger; Y Takahama; A Tarnok; Z Tian; G Toldi; J Tornack; E Traggiai; J Trotter; H Ulrich; M van der Braber; RAW Lier; M Veldhoen; S Vento-Asturias; P Vieira; D Voehringer; H-D Volk; K von Volkmann; A Waisman; R walker; MD Ward; K Warnatz; S Warth; JV Watson; C Watzl; L Wegener; A Wiedemann; J Wienands; G Willimsky; J Wing; P Wurst; L Yu; A Yue; Q Zhang; Y Zhao; S Ziegler; J Zimmermann

doi:10.1002/eji.201646632

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, H-D Chang, A Radbruch, M Akdis, I Andrae, F Annunziato, P Bacher, V Barnaba, L Battistini, WM Bauer, S Baumgart, B Becher, W Beisker, C Berek, A Blanco, G Borsellino, PE Boulais, RR Brinkman, M Buescher, DH BuschTP Bushnell, X Cao, A Cavani, PK Chattopadhyay, Q Cheng, S Chow, M Clerici, A Cooke, A Cosma, L Cosmi, A Cumano, VD Dang, D Davies, S De Biasi, G Del Zotto, S Della Bella, P Dellabona, G Deniz, M Dessing, A Diefenbach, J Di Santo, F Dieli, A Dolf, VS Donnenberg, T Doerner, GRA Ehrhardt, E Endl, P Engel, B Engelhardt, C Esser, B Everts, A Dreher, CS Falk, TA Fehniger, A Filby, S Fillatreau, M Follo, I Foerster, J Foster, GA Foulds, PS Frenette, D Galbraith, N Garbi, M Dolores Garcia-Godoy, J Geginat, K Ghoreschi, L Gibellini, C Goettlinger, CS Goodyear, A Gori, J Grogan, M Gross, A Gruetzkau, D Grummitt, J Hahn, Q Hammer, AE Hauser, DL Haviland, D Hedley, G Herrera, M Herrmann, F Hiepe, T Holland, P Hombrink, JP Houston, BF Hoyer, B Huang, CA Hunter, A Iannone, H-M Jaeck, B Javega, S Jonjic, K Juelke, S Jung, T Kaiser, T Kalina, B Keller, Salima Khan, D Kienhoefer, T Kroneis, D Kunkel, C Kurts, P Kvistborg, J Lannigan, O Lantz, A Larbi, S LeibundGut-Landmann, MD Leipold, MK Levings, V Litwin, Y Liu, M Lohoff, G Lombardi, L Lopez, A Lovett-Racke, Erik Lubberts, B Ludewig, E Lugli, HT Maecker, G Martrus, G Matarese, C Maueroeder, M McGrath, I McInnes, HE Mei, F Melchers, S Melzer, D Mielenz, K Mills, D Mirrer, J Mjosberg, J Moore, B Moran, A Moretta, L Moretta, TR Mosmann, S Mueller, W Muller, C Munz, G Multhoff, LE Munoz, KM Murphy, T Nakayama, M Nasi, C Neudoerfl, J Nolan, S Nourshargh, J-E O'Connor, W Ouyang, A Oxenius, R Palankar, I Panse, P Peterson, C Peth, J Petriz, D Philips, W Pickl, S Piconese, M Pinti, AG Pockley, MJ Podolska, C Pucillo, SA Quataert, TRDJ Radstake, B Rajwa, JA Rebhahn, D Recktenwald, EBM Remmerswaal, K Rezvani, LG Rico, JP Robinson, C Romagnani, A Rubartelli, B Ruckert, J Ruland, S Sakaguchi, F Sala-de-Oyanguren, Y Samstag, S Sanderson, B Sawitzki, A Scheffold, M Schiemann, F Schildberg, E Schimisky, SA Schmid, S Schmitt, K Schober, T Schueler, AR Schulz, T Schumacher, C Scotta, TV Shankey, A Shemer, A-K Simon, J Spidlen, AM Stall, R Stark, C Stehle, M Stein, T Steinmetz, H Stockinger, Y Takahama, A Tarnok, Z Tian, G Toldi, J Tornack, E Traggiai, J Trotter, H Ulrich, M van der Braber, RAW Lier, M Veldhoen, S Vento-Asturias, P Vieira, D Voehringer, H-D Volk, K von Volkmann, A Waisman, R walker, MD Ward, K Warnatz, S Warth, JV Watson, C Watzl, L Wegener, A Wiedemann, J Wienands, G Willimsky, J Wing, P Wurst, L Yu, A Yue, Q Zhang, Y Zhao, S Ziegler, J Zimmermann

Research output: Contribution to journal › Article › Academic › peer-review

373 Citations (Scopus)

Original language	Undefined/Unknown
Pages (from-to)	1584-1797
Number of pages	214
Journal	European Journal of Immunology
Volume	47
Issue number	10
DOIs	https://doi.org/10.1002/eji.201646632
Publication status	Published - 2017

Access to Document

10.1002/eji.201646632

http://hdl.handle.net/1765/102607

Cite this

Cossarizza, A., Chang, H-D., Radbruch, A., Akdis, M., Andrae, I., Annunziato, F., Bacher, P., Barnaba, V., Battistini, L., Bauer, WM., Baumgart, S., Becher, B., Beisker, W., Berek, C., Blanco, A., Borsellino, G., Boulais, PE., Brinkman, RR., Buescher, M., ... Zimmermann, J. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, 47(10), 1584-1797. https://doi.org/10.1002/eji.201646632

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author = "A Cossarizza and H-D Chang and A Radbruch and M Akdis and I Andrae and F Annunziato and P Bacher and V Barnaba and L Battistini and WM Bauer and S Baumgart and B Becher and W Beisker and C Berek and A Blanco and G Borsellino and PE Boulais and RR Brinkman and M Buescher and DH Busch and TP Bushnell and X Cao and A Cavani and PK Chattopadhyay and Q Cheng and S Chow and M Clerici and A Cooke and A Cosma and L Cosmi and A Cumano and VD Dang and D Davies and {De Biasi}, S and {Del Zotto}, G and {Della Bella}, S and P Dellabona and G Deniz and M Dessing and A Diefenbach and {Di Santo}, J and F Dieli and A Dolf and VS Donnenberg and T Doerner and GRA Ehrhardt and E Endl and P Engel and B Engelhardt and C Esser and B Everts and A Dreher and CS Falk and TA Fehniger and A Filby and S Fillatreau and M Follo and I Foerster and J Foster and GA Foulds and PS Frenette and D Galbraith and N Garbi and {Dolores Garcia-Godoy}, M and J Geginat and K Ghoreschi and L Gibellini and C Goettlinger and CS Goodyear and A Gori and J Grogan and M Gross and A Gruetzkau and D Grummitt and J Hahn and Q Hammer and AE Hauser and DL Haviland and D Hedley and G Herrera and M Herrmann and F Hiepe and T Holland and P Hombrink and JP Houston and BF Hoyer and B Huang and CA Hunter and A Iannone and H-M Jaeck and B Javega and S Jonjic and K Juelke and S Jung and T Kaiser and T Kalina and B Keller and Salima Khan and D Kienhoefer and T Kroneis and D Kunkel and C Kurts and P Kvistborg and J Lannigan and O Lantz and A Larbi and S LeibundGut-Landmann and MD Leipold and MK Levings and V Litwin and Y Liu and M Lohoff and G Lombardi and L Lopez and A Lovett-Racke and Erik Lubberts and B Ludewig and E Lugli and HT Maecker and G Martrus and G Matarese and C Maueroeder and M McGrath and I McInnes and HE Mei and F Melchers and S Melzer and D Mielenz and K Mills and D Mirrer and J Mjosberg and J Moore and B Moran and A Moretta and L Moretta and TR Mosmann and S Mueller and W Muller and C Munz and G Multhoff and LE Munoz and KM Murphy and T Nakayama and M Nasi and C Neudoerfl and J Nolan and S Nourshargh and J-E O'Connor and W Ouyang and A Oxenius and R Palankar and I Panse and P Peterson and C Peth and J Petriz and D Philips and W Pickl and S Piconese and M Pinti and AG Pockley and MJ Podolska and C Pucillo and SA Quataert and TRDJ Radstake and B Rajwa and JA Rebhahn and D Recktenwald and EBM Remmerswaal and K Rezvani and LG Rico and JP Robinson and C Romagnani and A Rubartelli and B Ruckert and J Ruland and S Sakaguchi and F Sala-de-Oyanguren and Y Samstag and S Sanderson and B Sawitzki and A Scheffold and M Schiemann and F Schildberg and E Schimisky and SA Schmid and S Schmitt and K Schober and T Schueler and AR Schulz and T Schumacher and C Scotta and TV Shankey and A Shemer and A-K Simon and J Spidlen and AM Stall and R Stark and C Stehle and M Stein and T Steinmetz and H Stockinger and Y Takahama and A Tarnok and Z Tian and G Toldi and J Tornack and E Traggiai and J Trotter and H Ulrich and {van der Braber}, M and RAW Lier and M Veldhoen and S Vento-Asturias and P Vieira and D Voehringer and H-D Volk and {von Volkmann}, K and A Waisman and R walker and MD Ward and K Warnatz and S Warth and JV Watson and C Watzl and L Wegener and A Wiedemann and J Wienands and G Willimsky and J Wing and P Wurst and L Yu and A Yue and Q Zhang and Y Zhao and S Ziegler and J Zimmermann",

year = "2017",

doi = "10.1002/eji.201646632",

language = "Undefined/Unknown",

volume = "47",

pages = "1584--1797",

journal = "European Journal of Immunology",

issn = "0014-2980",

number = "10",

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Cossarizza, A, Chang, H-D, Radbruch, A, Akdis, M, Andrae, I, Annunziato, F, Bacher, P, Barnaba, V, Battistini, L, Bauer, WM, Baumgart, S, Becher, B, Beisker, W, Berek, C, Blanco, A, Borsellino, G, Boulais, PE, Brinkman, RR, Buescher, M, Busch, DH, Bushnell, TP, Cao, X, Cavani, A, Chattopadhyay, PK, Cheng, Q, Chow, S, Clerici, M, Cooke, A, Cosma, A, Cosmi, L, Cumano, A, Dang, VD, Davies, D, De Biasi, S, Del Zotto, G, Della Bella, S, Dellabona, P, Deniz, G, Dessing, M, Diefenbach, A, Di Santo, J, Dieli, F, Dolf, A, Donnenberg, VS, Doerner, T, Ehrhardt, GRA, Endl, E, Engel, P, Engelhardt, B, Esser, C, Everts, B, Dreher, A, Falk, CS, Fehniger, TA, Filby, A, Fillatreau, S, Follo, M, Foerster, I, Foster, J, Foulds, GA, Frenette, PS, Galbraith, D, Garbi, N, Dolores Garcia-Godoy, M, Geginat, J, Ghoreschi, K, Gibellini, L, Goettlinger, C, Goodyear, CS, Gori, A, Grogan, J, Gross, M, Gruetzkau, A, Grummitt, D, Hahn, J, Hammer, Q, Hauser, AE, Haviland, DL, Hedley, D, Herrera, G, Herrmann, M, Hiepe, F, Holland, T, Hombrink, P, Houston, JP, Hoyer, BF, Huang, B, Hunter, CA, Iannone, A, Jaeck, H-M, Javega, B, Jonjic, S, Juelke, K, Jung, S, Kaiser, T, Kalina, T, Keller, B, Khan, S, Kienhoefer, D, Kroneis, T, Kunkel, D, Kurts, C, Kvistborg, P, Lannigan, J, Lantz, O, Larbi, A, LeibundGut-Landmann, S, Leipold, MD, Levings, MK, Litwin, V, Liu, Y, Lohoff, M, Lombardi, G, Lopez, L, Lovett-Racke, A, Lubberts, E, Ludewig, B, Lugli, E, Maecker, HT, Martrus, G, Matarese, G, Maueroeder, C, McGrath, M, McInnes, I, Mei, HE, Melchers, F, Melzer, S, Mielenz, D, Mills, K, Mirrer, D, Mjosberg, J, Moore, J, Moran, B, Moretta, A, Moretta, L, Mosmann, TR, Mueller, S, Muller, W, Munz, C, Multhoff, G, Munoz, LE, Murphy, KM, Nakayama, T, Nasi, M, Neudoerfl, C, Nolan, J, Nourshargh, S, O'Connor, J-E, Ouyang, W, Oxenius, A, Palankar, R, Panse, I, Peterson, P, Peth, C, Petriz, J, Philips, D, Pickl, W, Piconese, S, Pinti, M, Pockley, AG, Podolska, MJ, Pucillo, C, Quataert, SA, Radstake, TRDJ, Rajwa, B, Rebhahn, JA, Recktenwald, D, Remmerswaal, EBM, Rezvani, K, Rico, LG, Robinson, JP, Romagnani, C, Rubartelli, A, Ruckert, B, Ruland, J, Sakaguchi, S, Sala-de-Oyanguren, F, Samstag, Y, Sanderson, S, Sawitzki, B, Scheffold, A, Schiemann, M, Schildberg, F, Schimisky, E, Schmid, SA, Schmitt, S, Schober, K, Schueler, T, Schulz, AR, Schumacher, T, Scotta, C, Shankey, TV, Shemer, A, Simon, A-K, Spidlen, J, Stall, AM, Stark, R, Stehle, C, Stein, M, Steinmetz, T, Stockinger, H, Takahama, Y, Tarnok, A, Tian, Z, Toldi, G, Tornack, J, Traggiai, E, Trotter, J, Ulrich, H, van der Braber, M, Lier, RAW, Veldhoen, M, Vento-Asturias, S, Vieira, P, Voehringer, D, Volk, H-D, von Volkmann, K, Waisman, A, walker, R, Ward, MD, Warnatz, K, Warth, S, Watson, JV, Watzl, C, Wegener, L, Wiedemann, A, Wienands, J, Willimsky, G, Wing, J, Wurst, P, Yu, L, Yue, A, Zhang, Q, Zhao, Y, Ziegler, S & Zimmermann, J 2017, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies', European Journal of Immunology, vol. 47, no. 10, pp. 1584-1797. https://doi.org/10.1002/eji.201646632

TY - JOUR

T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies

AU - Cossarizza, A

AU - Chang, H-D

AU - Radbruch, A

AU - Akdis, M

AU - Andrae, I

AU - Annunziato, F

AU - Bacher, P

AU - Barnaba, V

AU - Battistini, L

AU - Bauer, WM

AU - Baumgart, S

AU - Becher, B

AU - Beisker, W

AU - Berek, C

AU - Blanco, A

AU - Borsellino, G

AU - Boulais, PE

AU - Brinkman, RR

AU - Buescher, M

AU - Busch, DH

AU - Bushnell, TP

AU - Cao, X

AU - Cavani, A

AU - Chattopadhyay, PK

AU - Cheng, Q

AU - Chow, S

AU - Clerici, M

AU - Cooke, A

AU - Cosma, A

AU - Cosmi, L

AU - Cumano, A

AU - Dang, VD

AU - Davies, D

AU - De Biasi, S

AU - Del Zotto, G

AU - Della Bella, S

AU - Dellabona, P

AU - Deniz, G

AU - Dessing, M

AU - Diefenbach, A

AU - Di Santo, J

AU - Dieli, F

AU - Dolf, A

AU - Donnenberg, VS

AU - Doerner, T

AU - Ehrhardt, GRA

AU - Endl, E

AU - Engel, P

AU - Engelhardt, B

AU - Esser, C

AU - Everts, B

AU - Dreher, A

AU - Falk, CS

AU - Fehniger, TA

AU - Filby, A

AU - Fillatreau, S

AU - Follo, M

AU - Foerster, I

AU - Foster, J

AU - Foulds, GA

AU - Frenette, PS

AU - Galbraith, D

AU - Garbi, N

AU - Dolores Garcia-Godoy, M

AU - Geginat, J

AU - Ghoreschi, K

AU - Gibellini, L

AU - Goettlinger, C

AU - Goodyear, CS

AU - Gori, A

AU - Grogan, J

AU - Gross, M

AU - Gruetzkau, A

AU - Grummitt, D

AU - Hahn, J

AU - Hammer, Q

AU - Hauser, AE

AU - Haviland, DL

AU - Hedley, D

AU - Herrera, G

AU - Herrmann, M

AU - Hiepe, F

AU - Holland, T

AU - Hombrink, P

AU - Houston, JP

AU - Hoyer, BF

AU - Huang, B

AU - Hunter, CA

AU - Iannone, A

AU - Jaeck, H-M

AU - Javega, B

AU - Jonjic, S

AU - Juelke, K

AU - Jung, S

AU - Kaiser, T

AU - Kalina, T

AU - Keller, B

AU - Khan, Salima

AU - Kienhoefer, D

AU - Kroneis, T

AU - Kunkel, D

AU - Kurts, C

AU - Kvistborg, P

AU - Lannigan, J

AU - Lantz, O

AU - Larbi, A

AU - LeibundGut-Landmann, S

AU - Leipold, MD

AU - Levings, MK

AU - Litwin, V

AU - Liu, Y

AU - Lohoff, M

AU - Lombardi, G

AU - Lopez, L

AU - Lovett-Racke, A

AU - Lubberts, Erik

AU - Ludewig, B

AU - Lugli, E

AU - Maecker, HT

AU - Martrus, G

AU - Matarese, G

AU - Maueroeder, C

AU - McGrath, M

AU - McInnes, I

AU - Mei, HE

AU - Melchers, F

AU - Melzer, S

AU - Mielenz, D

AU - Mills, K

AU - Mirrer, D

AU - Mjosberg, J

AU - Moore, J

AU - Moran, B

AU - Moretta, A

AU - Moretta, L

AU - Mosmann, TR

AU - Mueller, S

AU - Muller, W

AU - Munz, C

AU - Multhoff, G

AU - Munoz, LE

AU - Murphy, KM

AU - Nakayama, T

AU - Nasi, M

AU - Neudoerfl, C

AU - Nolan, J

AU - Nourshargh, S

AU - O'Connor, J-E

AU - Ouyang, W

AU - Oxenius, A

AU - Palankar, R

AU - Panse, I

AU - Peterson, P

AU - Peth, C

AU - Petriz, J

AU - Philips, D

AU - Pickl, W

AU - Piconese, S

AU - Pinti, M

AU - Pockley, AG

AU - Podolska, MJ

AU - Pucillo, C

AU - Quataert, SA

AU - Radstake, TRDJ

AU - Rajwa, B

AU - Rebhahn, JA

AU - Recktenwald, D

AU - Remmerswaal, EBM

AU - Rezvani, K

AU - Rico, LG

AU - Robinson, JP

AU - Romagnani, C

AU - Rubartelli, A

AU - Ruckert, B

AU - Ruland, J

AU - Sakaguchi, S

AU - Sala-de-Oyanguren, F

AU - Samstag, Y

AU - Sanderson, S

AU - Sawitzki, B

AU - Scheffold, A

AU - Schiemann, M

AU - Schildberg, F

AU - Schimisky, E

AU - Schmid, SA

AU - Schmitt, S

AU - Schober, K

AU - Schueler, T

AU - Schulz, AR

AU - Schumacher, T

AU - Scotta, C

AU - Shankey, TV

AU - Shemer, A

AU - Simon, A-K

AU - Spidlen, J

AU - Stall, AM

AU - Stark, R

AU - Stehle, C

AU - Stein, M

AU - Steinmetz, T

AU - Stockinger, H

AU - Takahama, Y

AU - Tarnok, A

AU - Tian, Z

AU - Toldi, G

AU - Tornack, J

AU - Traggiai, E

AU - Trotter, J

AU - Ulrich, H

AU - van der Braber, M

AU - Lier, RAW

AU - Veldhoen, M

AU - Vento-Asturias, S

AU - Vieira, P

AU - Voehringer, D

AU - Volk, H-D

AU - von Volkmann, K

AU - Waisman, A

AU - walker, R

AU - Ward, MD

AU - Warnatz, K

AU - Warth, S

AU - Watson, JV

AU - Watzl, C

AU - Wegener, L

AU - Wiedemann, A

AU - Wienands, J

AU - Willimsky, G

AU - Wing, J

AU - Wurst, P

AU - Yu, L

AU - Yue, A

AU - Zhang, Q

AU - Zhao, Y

AU - Ziegler, S

AU - Zimmermann, J

PY - 2017

Y1 - 2017

U2 - 10.1002/eji.201646632

DO - 10.1002/eji.201646632

M3 - Article

SN - 0014-2980

VL - 47

SP - 1584

EP - 1797

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 10

ER -