Haemoglobin staining for in vivo portraying of functional vasculature in experimental zebrafish embryos

Pingpin Zheng; Lies-anne Severijnen; Rob Willemsen; J.M. Kros

doi:10.1016/j.bbrc.2009.01.175

Haemoglobin staining for in vivo portraying of functional vasculature in experimental zebrafish embryos

Pingpin Zheng, Lies-anne Severijnen, Rob Willemsen, J.M. Kros

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta. posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments. (C) 2009 Elsevier Inc. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	823-824
Number of pages	2
Journal	Biochemical & Biophysical Research Communications
Volume	380
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.01.175
Publication status	Published - 2009

Research programs

EMC MGC-02-96-01
EMC MM-03-24-01
EMC MM-03-44-06

Access to Document

10.1016/j.bbrc.2009.01.175

http://hdl.handle.net/1765/24287

Cite this

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title = "Haemoglobin staining for in vivo portraying of functional vasculature in experimental zebrafish embryos",

abstract = "Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta. posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments. (C) 2009 Elsevier Inc. All rights reserved.",

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AU - Zheng, Pingpin

AU - Severijnen, Lies-anne

AU - Willemsen, Rob

AU - Kros, J.M.

PY - 2009

Y1 - 2009

N2 - Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta. posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments. (C) 2009 Elsevier Inc. All rights reserved.

AB - Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta. posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments. (C) 2009 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.bbrc.2009.01.175

DO - 10.1016/j.bbrc.2009.01.175

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